12份白花泡桐材料亲缘关系的ISSR分析.doc
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1、12份白花泡桐材料亲缘关系的ISSR分析ISSR Analysis of Relationship of 12 Accessions of Paulownia fortunei ZHENG Xinghua ZHENG Zhenglian DING Hui XIAO Shaohua QIN Zihai (Guangxi Futong Forestry Science and Technology Co., Ltd., Nanning, Guangxi 530021) Abstract The genetic relationship of 12 accessions of Paulownia f
2、ortunei including two clonesFu Tong, Lv Tong clones and 10 individuals of different provenances was analyzed by ISSR data and clustered with DPSv3.01. The results showed that a total of 66 DNA bands were obtained, of which 37 bands were polymorphic and the percentage of polymorphic bands was 0.561.
3、All these accessions could be divided into five groups by ISSR cluster analysis. The first group consisted of clone Fu Tong and individuals of Henan and Hebei provenances; the second group included clone Lv Tong and the individuals of Hunan, Hubei and Jiangsu provenances; the third group the individ
4、uals of Zhejiang and Guangdong provenances; the fourth group the individuals of Jiangxi and Fujian provenances; and the fifth group the individuals of Guangdong provenances. It is hence concluded that clonesFu Tong and Lv Tong are different. Keywords Paulownia fortunei ; genetic relationship ; inter
5、-simple sequence repeat 泡桐(Paulownia)?橹泄?最重要的速生优质用材和生态防护树种之一,在中国林业生产和生态环境保护方面一直发挥着不可替代的作用。中国泡桐种质资源分布广泛,种类丰富,仅大陆就有9种2变种和众多变异类型1。白花泡桐(Paulownia fortunei)为玄参科(Scrophulariaceae)泡桐属落叶乔木,高达20 m,在中国分布于10多个省(区)。具有耐盐碱、抗性强、对气候适应范围广、生长快、材质优异、生态价值高等特点,为中国中东部地区的重要速生造林树种2,具有很大的发展潜力。 为开发利用泡桐资源,国家林业局专门成立了泡桐研究开发中心,收
6、集泡桐优良种质资源,选育泡桐优良无性系,在林分结构、经营密度、干形培育等定向培育方面取得了较大进展。同时以泡桐木材为基材,研发磁控溅射镀膜木材技术,为泡桐木材的利用及增值奠定了基础,有利于整个泡桐产业的提质增效。 除国家层面外,河南、江西、山东、贵州等省份也有泡桐研究及开发利用的相关报告。在广西,目前主推的泡桐品种有福桐、绿桐等,但其来源地不甚清楚。为此,本研究开展研究摸清其来源,为其造林推广提供参考信息。 ISSR(inter-simple sequence repeat)分子标记是由zietkiewiez等提出的一种新型分子标记,是在SSR(simple sequence repeat)标
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