IMRT、VMAT模式照射离体人肺癌细胞的生物学效应研究.doc
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1、IMRT、VMAT模式照射离体人肺癌细胞的生物学效应研究Abstract Objective To observe the biological effects of intensity-modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT) irradiation for the human lung cancer cells in vitro, and to study the mechanism of IMRT and VMAT irradiation, in order to provide e
2、vidence for formulating reasonable clinical radiotherapy plan. Methods The human lung squamous carcinoma cells SK-MES-1 and human lung small cell cancer cells NCI-H446 were selected to establish tumor model by agar suspension culture method in vitro, then the cells were given rapid radiation researc
3、h at 9 dose levels. After irradiation for 3 min, the survival percentage of cells was calculated, GraphPad Prism software was used to process data, the cell survival curve was fit with linear-quadratic formula, then the SK-MES-1, NCI-H446 at exponential growth phase were divided into four groups: VM
4、AT irradiation group (the energy of linear accelerator was 6 MV, for 6 min); IMRT irradiation for 15 min group; IMRT irradiation for 30 min group; IMRT irradiation for 45 min group. The total irradiation of each group was 8 Gy, once a day, 2 Gy every time, finished in 4 days. At last, the cloning as
5、say was used to calculate the survival rate of cells, to compare the radiation characteristics and biological effects between IMRT model and VMAT model. Results After finishing rapid radiation, the radiobiological parameters of SK-MES-1, NCI-H446 cells were achieved. The detailed numerical value of
6、each parameter of SK-MES-1 cells (D0, Dq, N value, value, value, / value) was 0.87 Gy, 0.48 Gy, 3.6, 0.17 Gy-1, 0.091 Gy-2, 1.87 Gy respectively; which of NCI-H446 cells was 0.61 Gy, 0.35 Gy, 4.0, 0.98 Gy-1, 0.089 Gy-2, 11.01 Gy respectively. In the irradiation of 4 d with 8 Gy, the survival rate of
7、 SK-MES-1 cells in all groups (VMAT irradiation group and IMRT irradiation for 15, 30, 45 min group) was 7.25%, 8.95%, 9.63%, 11.32% respectively, the survival rate under the IMRT irradiation was higher than that of VMAT irradiation. The survival rate of NCI-H446 cells in the groups above was 0.192%
8、, 0.205%, 0.208%, 0.209% respectively, the difference was not obvious. Conclusion The cellular radiosensitivity (D0, N value) of human lung squamous carcinoma cells SK-MES-1 is lower than that of human lung small cell cancer cells NCI-H446, the sublethal damage repair capacity (Dq value and / value)
9、 is stronger than that of NCI-H446, the changes of dosage rates has significant influence for the radiobiological effect, the prolonged single dose output time of IMRT model lead to the increase the survival rate of SK-MES-1 cells, without obvious increase in NCI-H446 cells. Key words Human lung can
10、cer cells; Intensity-modulated radiotherapy; Volumetric modulated arc therapy; Biological effects 肺癌(1ung cancer)的发病率及死亡率呈不断上升趋势,在20世纪末已成为各种癌症死亡的首要原因,对人类健康的威胁越来越严重1。大部分肺癌患者确诊时已经处于中晚期或晚期,不可以接受手术治疗,只能选择以放射治疗为主的综合治疗模式2-3。因此,研究寻找非小细胞肺癌患者出现放射抗拒性的规律,寻找放射增敏的方法是提高治愈率的途径之一。而肺癌细胞的生物学效应变化情况对于疾病的发展转归有积极的反应价值4-5
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- 关 键 词:
- IMRT VMAT 模式 照射 离体人 肺癌 细胞 生物学 效应 研究
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