不同来源的细胞因子诱导的杀伤细胞体外抗肿瘤作用的比较研究.doc
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1、不同来源的细胞因子诱导的杀伤细胞体外抗肿瘤作用的比较研究Abstract Objective To compare difference of the anti-tumor response of CIK cells from peripheral blood of patients with lung cancer and umbilical cord blood in vitro. Methods Peripheral monocytes were isolated from patients with lung cancer, and cord blood monocytes from
2、healthy donors. Isolated cells were cultured and induced as CIK cells in vitro. The anti-tumor activities were measured by MTT assay and flow cytometry. Intracellular staining was used to test the secretion of cytokines. Results The percentage of CD3+, CD3+CD56+ and CD3+CD8+ cells in cord blood CIK
3、and peripheral blood CIK of patients with lung cancer was increased with the prolongation of culture time, and there was no significant difference between the two groups; After cultured for 21 days, the proliferation rate of cord blood CIK cells and lung cancer patients peripheral blood CIK cells wa
4、s(185.7639.68)% and(254.3274.60)%, respectively, and there was significant difference between the two group (P=0.0012); The anti-tumor activity of cord blood CIK cells was higher than that of lung cancer patients peripheral blood CIK cells(effector-target ratio of 10:1, when the target cells were K5
5、62 cells P=0.0016; when the target cells were A549 cells P=0.0022). CD107a expression in cord blood CIK cells was higher than CIK cells from the peripheral blood of patients with lung cancer(when the target cells were K562 cells, P=0.0469; when the target cells were A549 cells, P=0.0413); Secretion
6、levels of IFN- and TNF- in cord blood CIK cells were higher than that of CIK cells from lung cancer patients(P=0.0352, P=0.0389); The percentage of apoptotic K562 cells induced by cord blood CIK and CIK cells from lung cancer patients was (25.304.87)% and (19.875.41)%,respectively. There was no sign
7、ificant difference between them(P=0.2658). Conclusion The phenotype, function and mechanism of action of CIK cells can be detected simultaneously by flow cytometry. This results can provide experimental evidence for clinical application of CIK cells. Key words Cytokine-induced killer cells; Flow cyt
8、ometry; Immunotherapy; CD107a 细胞因子诱导的杀伤细胞(cytokine induced killer cells,CIK)是一群异质性的具有抗肿瘤作用的细胞。CIK细胞主要表达CD3和CD56,抗肿瘤活性强,增殖迅速。用患者自身PBMC诱导的CIK细胞已经显示良好的临床疗效,尤其对慢性髓系白血病、肝癌、结直肠癌、肾癌、肺癌、乳腺癌等1。但是肿瘤患者在经历了放化疗后,经常出现白细胞减少、贫血等症状。尤其晚期恶性肿瘤患者每毫升外周血中单核细胞的数量也较正常人少。因此为了减轻患者负担,提高临床疗效,本研究比较了来自健康捐助者的脐血CIK细胞和来自肺癌患者外周血的CIK细
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- 不同 来源 细胞因子 诱导 杀伤 细胞 体外 肿瘤 作用 比较 研究
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