最新考研资料:北京大学细胞生物学第六章基质与内膜下-PPT文档.ppt
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1、vMilstein et al: Studying the synthesis of light chain of IgG (in cell-free systems, 20 Aa longer at N-terminal end than the authentic light chain ) Adding ER membranes to this system leads to the production of an IgG light chain of the correct size. vA Schematic model for the synthesis of a secreto
2、ry protein on a membrane-bound ribosome of the rough ER Signal-recognition particle, SRP: Six different polypeptides complexed with a 300-nucleotide (7S)molecule of RNA. ER signal sequence: Typically 15-30 amino acids: Consist of three domains: a positively charged N-terminal region, a central hydro
3、phobic region, and a polar region adjoining the site where cleavage from the mature protein will take place. A signal sequence on nascent seretory proteins targets them to the ER and is then cleaved off SRP receptor (GTP binding protein) SRP have three main active sites: One that recognizes and bind
4、s to ER signal sequence; One that interacts with the ribosome to block further translation; One that binds to the ER membrane (docking protein) The sorting signal of VSV glycoproteins : Asp-X-Gln或DXE Figure 6-43. The sorting of proteins destined for the apical and basolateral plasma membranes of epi
5、thelial cells. When cultured MDCK cells are infected simultaneously with VSV and influenza virus, the VSV glycoprotein is found only on the basolateral membrane, whereas the HA glycoprotein of the influenza virus is found only on the apical membrane. Like these viral proteins, some cellular proteins
6、 are sorted directly to the apical membrane and others to the basolateral membrane via specific transport vesicles that bud from the trans-Golgi network. In certain other polarized cells, some apical and basolateral proteins are transported together to the basolateral surface; the apical proteins th
7、en move selectively, by endocytosis and transcytosis, to the apical membrane. K. Simons et al., Cell 62:207; K. Mostov et al., JCB. 116:577 vStart-transfer Sequence Sar- GDP dissociates from the ER Antibodies is able to block the budding of vesicle from ER but have no effect on vesicle transport fro
8、m one Golgi compartment to another in mammalian cell. v COPI-coated vasicles transporting Escaped ER resident Proteins Back to the ER. (1) The assembly of a COPI-coat is mediated by ADP- ribosylation factor(ARF), GTP binding protein, which is required for vesicle transfer between cisternae. (2) COPI
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