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1、汇 报 人:宁漂 2015.12.21,Novel Mussel-Inspired Injectable Self-Healing Hydrogel with Anti-Biofouling Property,contents,background proteins or microorganisms could easily adhere to implanted hydrogels and form biofouling films, not only blocking the circulation of loaded biomolecules but also triggering a
2、n immune response or inflammation. Mussel-Inspired Marine mussels secrete foot proteins which after a curing process can form byssus consisting of proteinaceous thread and adhesive plaque to adhere to various substrates underwater,The self-repair of mussel byssal threads is mainly attributed to the
3、reversible metal-catechol coordination between metals like Fe 3+ and catechol groups from an amino acid called 3,4-dihydroxyphenyl- L -alanine (DOPA), and other interactions like cation- interaction can also play a role.,1 Introduction,2 Synthesis,3 Characteristic,Temperature-responsive storage and
4、To rule out the possibility that thermal-induced gelation was due to cathechols coupling reactions after oxidation.,3 Characteristic,essential of the hydrogel,3 Characteristic,Theself-healing mechanism,3 Characteristic,fluorescence microscopy images of uncoated and DNODN hydrogel coated microwell di
5、shes after exposureto Caco-2 cells for 48 h are shown to demonstrate exceptional antifouling performance against cell attachment of The DNODN hydrogel。,1、 we have developed a novel injectable thermosensitive self-healing hydrogel DNODN with anti-biofouling property.,2、 The thermosensitive property a
6、nd shearing thinning behavior of the hydrogel allow it injectable in vivo at body temperature. 3 、the anti-biofouling properties can effectively inhibit the formation of biofilms. 4、The self-mending DNODN hydrogel can withstand high strain and repeated deformation and quickly recover its mechanical
7、properties 5、 structure through the catechol-mediated reconstruction of hydrogen bonding and aromatic interactions, thus reducing the infl ammation risk in bioengineering applications,4 conclusions,Thanks,4 conclusions,实验条件 特征黏度:1.86dL/g 浸渍浓度:10、30、 50、 70、90、110mg/L 浸渍时间:15min 浸渍温度:20 作用对象:金黄色葡萄球菌
8、单层纱布 染菌24h,4.1 PDADMAC浸渍浓度的影响,4 实验结果,4 实验结果,浸渍时间、浸渍温度、烘干条件一定时,纱布抗菌率随着 PDADMAC浸渍浓度增大而提高。 当浸渍浓度为50mg/L时,抗菌率已达到96.75%。因此选择50mg/L进行下步实验,以期降低纱布的制备成本。,实验条件 特征黏度:1.86dL/g 浸渍浓度:50mg/L 浸渍时间:15、30、 60、90、120min 浸渍温度:20 作用对象:金黄色葡萄球菌 单层纱布 染菌24h,4 实验结果,4.2 纱布浸渍时间的影响,4 实验结果,浸渍浓度、浸渍温度、烘干条件一定时,纱布的抗菌率随着浸渍时间的增加而提高。 当
9、浸渍时间超过30min时,抗菌率的增幅已趋于平缓。为了节约时间,提高生产效率,选择30min为较佳浸渍时间。,实验条件 特征黏度:1.86dL/g 浸渍浓度:50mg/L 浸渍时间:30min 浸渍温度:20、40、 60、80、100 作用对象:金黄色葡萄球菌 单层纱布 染菌24h,4 实验结果,4.3 纱布浸渍温度的影响,4 实验结果,抗菌纱布制备工艺参数,浸渍浓度、浸渍时间、烘干条件一定时,纱布的抗菌率随着浸渍温度提高而显著增加。 但是,浸渍温度过高时,会造成能源浪费。因此,选择60为较佳浸渍温度。,4.4 纱布的透湿性测定,4 实验结果,fluorescence microscopy
10、images of uncoated and DNODN hydrogel coated microwell dishes after exposureto Caco-2 cells for 48 h are shown to demonstrate exceptional antifouling performance against cell attachment of The DNODN hydrogel。,实验条件 特征黏度: 1.86、2.60、3.35dL/g 浸渍浓度:100、150、200、250 mg/L 浸渍时间:30min 浸渍温度:30 单层纱布,4.5 PDADMAC
11、特征黏度的影响,在一定浸渍浓度下,PDADMAC的特征黏度越大,纱布抗菌率越高,特征黏度对纱布抗菌率的影响较大。,4 实验结果,实验条件 特征黏度: 1.86、2.60、 3.35dL/g 浸渍浓度:50mg/L 浸渍时间:30min 浸渍温度:60 作用对象:金黄色葡萄球菌 单层纱布 染菌24h,4.6 纱布使用时间的影响,纱布抗菌率随着使用时间的增加而增大,并且含PDMDAAC的抗菌纱布具有较好的抗菌作用持久性和快速性。,4 实验结果,实验条件 特征黏度: 1.86dL/g 浸渍浓度:50mg/L 浸渍时间:30min 浸渍温度:60 作用对象:金黄色葡萄球菌 单层纱布 染菌1、8、16、24h,4.7 纱布使用层数的影响,纱布使用层数对其抗菌效果有一定影响,抗菌率随着使用层数增加而增加。纱布层数过多时,病人会有不舒适感。因此,根据实际情况选择合适的使用层数十分必要。,4 实验结果,实验条件 特征黏度: 1.86dL/g 浸渍浓度:50mg/L 浸渍时间:30min 浸渍温度:60 作用对象:金黄色葡萄球菌 1、3、5、7层纱布 染菌24h,抗菌纱布使用参数,非常感谢赵晓蕾老师在科研训练过程中对我们小组的悉心指导。虽然遇到各种困难,但在老师指导下,最终顺利完成实验内容,在此表示感谢。 汇报完毕,感谢在座的老师和同学们,请指正。,致 谢,Thanks,
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