复制第三讲.ppt
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1、* The position on the DNA at which replication start points are found. (4 methods discussed) * A DNA sequence that when added to a non-replicating DNA causes it to replicate. * A DNA sequence whose mutation abolishes replication. * A DNA sequence that in vitro is the binding target for enzyme comple
2、xes known to function in initiation of DNA replication. Characteristics used to define Origins of Replication 免疫学信息网 http:/ 1) In synchronously replicating cells, density transfer experiments can reveal which sequences replicate earliest. What is the direction of fork movement here? 免疫学信息网 http:/ 2)
3、 Electron microscopy of bubbles after restriction enzyme digestion 免疫学信息网 http:/ 免疫学信息网 http:/ 3) Hybridizing lagging strand nascent DNA to strand-specific fragments shows site of switch from leading to lagging strand synthesis 免疫学信息网 http:/ 4) 2D Gel electrophoresis of replication intermediates fol
4、lowed by hybridization with a DNA fragment reveals whether a replication bubble originates in the fragment Dimension 1: separates by size; dimension 2: separates by shape 免疫学信息网 http:/ Sequence that when added to a non-replicating DNA causes it to replicate; this approach was used to identify Autono
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