参与原核生物DNA复制的酶类和蛋白质EnzymesandProteins.ppt
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1、参与原核生物DNA复制的 酶类和蛋白质 Enzymes and Proteins Involved in DNA Replication in Prokaryotes 高方远 马欣荣 康海岐,DNA replication(bacteria) Initiation Elongation Termination Daughter DNA partition,* the origin of replication is defined * replication bubble. * Replication fork * Unidirectional or bidirectional.,Origin
2、s,Elongation(semidiscontinuous),termination,参与DNA复制的酶类,1、DNA聚合酶 2、DNA引发酶(DNA primase) 3、DNA连接酶 4、与DNA几何学性质相关的酶,I polA major repair enzyme II polB minor repair enzyme III polC replicase IV dinB SOS repair V umuC、D SOS repair,DNA polymerases in E.coli,DNA Polymerase I,DNA polymerase 3 5 exonuclease 5
3、3 exonuclease,DNA Polymerase I,Subunit composition of E.coli DNA polymerase III holoenzyme,E.coli Pol III Beta-subunit,Fig. 1. Model of SOS translesion replication by DNA polymerase V. The two DNA strands are shown as green lines, and the replication-blocking lesion is represented by the red rectang
4、le. The three major steps in TLR are pre-initiation (2), in which the RecA nucleoprotein filaments assembles; initiation (3 and 4), which involves binding of pol V to the primer-template and loading of the subunit clamp; and lesion bypass by pol V holoenzyme (5). SSB is suggested to help in displaci
5、ng RecA from DNA both at the initiation and lesion bypass steps.,2、DNA引发酶(DNA primase),Use host RNA polymerase as primase (M13) primosome primase (dnaG protein) (E.coli) other proteins X174: only primase, without the other proteins,DNA Polymerase I,与DNA几何学性质相关的酶,解旋酶(Helicase) 拓扑异构酶(Topoisomerases),解
6、旋酶(Helicase),至少4种helicases * rep helicase * DNA helicase II * DNA helicase III * dnaB Protein: 在E.coli DNA复制中解开 DNA双链,拓扑异构酶(Topoisomerases),拓扑异构酶I(topA gene) act on highly negatively supercoiled DNA stabilize single-stranded regions,Figure 14.16 Bacterial type I topoisomerases recognize partially un
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