AS 5013-24-1-2009 Food microbiology Method 24-1 Microbiology of food and animal feeding stuffs—Horizontal method for the detection and.pdf
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1、 i AS 5013.24.12009 www.standards.org.au Australian Standard Food microbiology Method 24.1: Microbiology of food and animal feeding stuffsHorizontal method for the detection and enumeration of Listeria monocytogenesDetection method (ISO 11290-1:1996, MOD) PREFACE This Standard was prepared by the St
2、andards Australia Committee FT-024, Food Products, and Constituted Subcommittee FT-024-01, Food Microbiology, to supersede in part AS/NZS 1766.2.151998, Food microbiology, Method 2.15: Examination of specific organismsListeria monocytogenes in dairy products and AS/NZS 1766.2.16.11998, Food microbio
3、logy, Method 2.16.1: Examination for specific organismsFood and animal feeding stuffsHorizontal method for the detection and enumeration of Listeria monocytogenesDetection method. This Standard is an adoption with national modifications and reproduced from ISO 11290- 1:1996, Microbiology of food and
4、 animal feeding stuffsHorizontal method for the detection and enumeration of Listeria monocytogenes, Part 1: Detection method including Amendment 1:2004, which is added at the end of the ISO text. ISO 11290-1:1996 was confirmed on 20 June 2005. Additional requirements for Australian conditions are l
5、isted in Appendix ZZ. The objective of this revision is to incorporate the ISO Amendment 1:2004, which modifies the isolation media and the haemolysis test, and adds precision data. As this Standard is reproduced from an International Standard, the following applies: (a) Its number appears on the co
6、ver and title page while the International Standard number appears only on the cover. (b) In the source text this part of ISO 11290 should read this Australian Standard. (c) A full point substitutes for a comma when referring to a decimal marker. (d) Substitute mL for ml whenever it appears. Referen
7、ces to International Standards should be replaced by references to Australian Standards as follows: AS 5013.24.12009 ii Reference to International Standard Australian Standard ISO AS 6887 MicrobiologyGeneral guidance for the preparation of dilutions for microbiological examination 5013 5013.11.1 Foo
8、d microbiology Method 11.1: Microbiology of food animal feeding stuffPreparation of test samples initial suspension and decimal dilutions for microbiological examination General rules for the preparation of the initial suspension and decimal dilutions 7218 MicrobiologyGeneral guidance for microbiolo
9、gical examination 5013.14 Method 14: Microbiology of food and animal feeding stuffsGeneral rules for microbiological examinations The laboratory should have a clearly defined quality control system to ensure that the apparatus, culture media, reagents and technique are suitable for the test. The use
10、 of positive controls is part of this system. The terms normative and informative have been used in this Standard to define the application of the annex or appendix to which they apply. A normative annex or appendix is an integral part of a Standard, whereas an informative annex or appendix is only
11、for information. ii ii The Standard is downloaded from Standard Sharing iii CONTENTS IS011290=1:1996(E) Contents Page 1 Scope 2 Normative references 3 Definitions 4 Principle . 5 Culture media and reagents 6 Apparatus and glassware 7 Sampling 8 Preparation of test sample . 9 Procedure 10 Expression
12、 of results . 11 Test report . Annexes 1 1 1 2 2 3 3 3 3 7 7 A Diagram of procedure 8 B Composition and preparation of culture media and reagents 9 C Henry illumination test . 16 0 IS0 1996 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in
13、any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization Case Postale 56 l CH-1211 Geneve 20 l Switzerland Printed in Switzerland ii iii iii iv INTRODUCTION IS011290=1:19
14、96(E) 0 IS0 Introduction Because of the large variety of food and feed products, this horizontal method may not be appropriate in every detail for certain products for which it may be necessary to use different or specific methods. Neverthe- less, in all cases, every attempt should be made to apply
15、this horizontal method as far as possible and that deviations from this will only be made if absolutely necessary for justified technical reasons. When this part of IS0 11290 is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal metho
16、d has been followed and the reasons for deviations from it in the case of particular products. The harmonization of test methods cannot be immediate, and for certain groups of products International Standards and/or national standards may already exist that do not comply with this horizontal method.
17、 It is hoped that when such standards are reviewed they will be changed to comply with this part of IS0 11290 so that eventually the only remaining departures from this horizontal method will be those necessary for well-established techni- cal reasons. IV iv iv The Standard is downloaded from Stand
18、ard Sharing 1 INTERNATIONAL STANDARD IS0 IS0 11290-I : 1996(E) Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Part 1: Detection method WARNING - In order to safeguard the health of laboratory personnel, it is strongly
19、recommended that tests for detecting Listeria monocytogenes are undertaken in properly equipped laboratories, under the control of a skilled microbiologist, and that great care is taken in the disposal of all incubated materials. In particular, it is strongly recommended that pregnant personnel do n
20、ot manipulate cultures of L. monocyto- genes. 1 Scope This part of IS0 11290 specifies a horizontal method for the detection of Listeria monocytogenes. Subject to the limitations discussed in the introduction, this part of IS0 11290 is applicable to products in- tended for human consumption or anima
21、l feeding. 2 Normative references The following standards contain provisions which, through reference in this text, constitute provisions of this part of IS0 11290. At the time of publication, the editions indicated were valid. All standards are subject to revision, and parties to agreements based o
22、n this part of IS0 11290 are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of IEC and IS0 maintain registers of currently valid International Stan- dards. IS0 6887:1983, Microbiology - General guidance for the preparation of
23、dilutions for microbiological exam- ina tion. IS0 7218: 1996, Microbiology of food and animal feed- ing stuffs - General rules for microbiological exam- ina tions. 3 Definitions For the purposes of this part of IS0 11290, the follow- ing definitions apply. 3.1 Lis teria monocytogenes: Microorganisms
24、 which form typical colonies on solid selective media and which display the morphological, physiological and biochemical characteristics described when tests are carried out in accordance with this part of IS0 11290. 3.2 detection of Listeria monocytogenes: Deter- mination of the presence or absence
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