异种角膜基质异位植入后免疫病理学研究.pdf
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1、实验研究 异种角膜基质异位植入后免疫病理学研究 陈根云 吴 静 徐锦堂 赵松滨 Immunopathologic study on fresh and dehydrated xenografts in porcine- to-mouse model of corneal stroma hetertopic xenotransplantation Chen Genyun, Wu Jing, Xu Jintang, Zhao Songbin. Department of Ophthalmology, Medical College of Jinan University, Guangzhou 51
2、0632, China Abstract Objective To observe the immunopathologic alterations of the fresh and dehydrated xenografts in a porcine-to- mouse model of corneal stroma hetertopic xenotransplantation and assess its immunocompatibility. Methods Twenty-four mice were randomly divided into 4 groups. The fresh
3、and dehydrated corneal stroma from porcine corneas were subcutaneously implanted into the mice of experimental groups, and xenogeneic dermas implantation mice and sham operation mice served as positive control and negative control group, respectively. Grafted corneal stroma was excised 30 days after
4、 grafting for histopathologic study, and the changes of total T lymphocyte and its subset counts in the xenografts were observed using the direct immunofluoresecent technique. Results No edema and ulceration were seen in the graft area in experiment groups 1 month after surgery by clinical observati
5、on. A small quantities of lymphocyte, macrophages, CD3 ( + )T cells, CD4 ( + )T cells and CD8 ( + )T cells infiltrated into both of the fresh and dehydrated xenografts at month 1 in postimplantation, but number of infiltrated cells (79. 00 9. 78 in fresh xenograft group, and 71. 83 9. 54 in dehydrat
6、ed xenograft group) was less than that of xenogeneic dermas group (134. 50 19. 71) (P 0. 05) ;(3) 3 个组的 CD4 + / CD8 + 的统计学处理结果与 CD4 + 淋 巴细胞数的处理结果一致。 91 眼科研究 2006 年 2 月第 24 卷第 1 期 表 1 术后 1 个月植入材料内浸润 的淋巴细胞计数结果(x s) Table 1 Numbers of T cells infiltrated into three kinds of xenografts 1 month in postop
7、eration (x s, n =6) GroupCD4 +CD8 +CD4 + / CD8 + Xenogeneic dermas134.50 19.7165.67 8.122.05 0.17 Fresh xenografts79.00 9.78b59.33 10.37a1.35 0.20b Dehydrated xenografts71.83 9.54b61.50 6.47a1.17 0.12b F36.840.8746.30 P0.000.440.00 aP 0. 05,bP 0. 05 vs Xenogeneic dermas group (one-way ANOVA, SNK-q t
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- 关 键 词:
- 角膜 基质 植入 免疫 病理学 研究
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