BS-7857-3-1997 ISO-13722-1996.pdf
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1、BRITISH STANDARD BS 7857-3:1997 ISO 13722:1996 Methods for Microbiological examination of meat and meat products Part 3: Enumeration of Brochothrix thermosphacta Colony-count technique ICS 07.100.30 Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00
2、:00 2006, Uncontrolled Copy, (c) BSI BS 7857-3:1997 This British Standard, having been prepared under the direction of the Consumer Products and Services Sector Board, was published under the authority of the Standards Board and comes into effect on 15 April 1997 BSI 11-1998 ISBN 0 580 27236 2 Natio
3、nal foreword This British Standard reproduces verbatim ISO 13722:1996 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology, which has the responsibility to: aid enquirers to understand the text; present to the
4、responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organizations represented on this committee can be obtained
5、on request. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or using the “Find” facility of the BSI Standa
6、rds Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of page
7、s This document comprises a front cover, an inside front cover, pages i and ii, the ISO title page, pages ii to iv, pages 1 to 4, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendme
8、nt table on the inside front cover. Amendments issued since publication Amd. No.DateComments Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7857-3:1997 BSI 11-1998i Contents Page National forewordInside fro
9、nt cover Forewordii Text of ISO 137221 Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Uncontrolled Copy, (c) BSI ii blank Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Unc
10、ontrolled Copy, (c) BSI Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7857-3:1997 ii BSI 11-1998 Contents Page Forewordii 1Scope1 2Normative references1 3Definition1 4Principle1 5Dilution fluid, culture me
11、dia and reagents1 6Apparatus2 7Sampling3 8Preparation of test sample3 9Procedure3 10Expression of results3 11Precision4 12Test report4 Annex A (informative) BibliographyInside back cover Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Un
12、controlled Copy, (c) BSI BS 7857-3:1997 BSI 11-1998iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees.
13、 Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Interna
14、tional Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bod
15、ies casting a vote. International Standard ISO 13722 was prepared by Technical Committee ISO/TC 34, Agricultural food products, Subcommittee SC 6, Meat and meat products. Annex A of this International Standard is for information only. Descriptors: Agricultural products, food products, animal product
16、s, meat, poultry meat, meat products, tests, microbiological analysis, counting, Brochothrix thermosphacta, bacteria count methods. Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Uncontrolled Copy, (c) BSI iv blank Licensed Copy: London
17、 South Bank University, London South Bank University, Fri Dec 08 10:32:00 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7857-3:1997 BSI 11-19981 1 Scope This International Standard specifies a method for the enumeration of viable Brochothrix thermosphacta in all kinds of meat and meat products, incl
18、uding poultry, by means of a colony-count technique. 2 Normative references The following standards contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publication, the editions indicated were valid. All standards are subject
19、 to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid International Standards. ISO 3100-2:1988, Mea
20、t and meat products Sampling and preparation of test samples Part 2: Preparation of test samples for microbiological examination. ISO 6887:1993, Microbiology General guidance for the preparation of dilutions for microbiological examination. ISO 7218:1996, Microbiology of food and animal feeding stuf
21、fs General rules for microbiological examinations. 3 Definition For the purposes of this International Standard, the following definition applies. 3.1 brochothrix thermosphacta gram-positive bacteria which form characteristic oxidase-negative colonies on a solid selective medium streptomycin sulfate
22、/thallium acetate/actidione (STAA) agar under the test conditions specified in this International Standard 4 Principle 4.1 Surface plating, on a solid selective culture medium contained in Petri dishes, of a specified quantity of the test sample if the initial product is liquid, or of a specified qu
23、antity of the initial suspension in the case of other products. Preparation of other plates, under the same conditions, using decimal dilutions of the test sample or of the initial suspension. 4.2 Incubation of the plates between 22 C and 25 C for 48 h 4 h. 4.3 Subjection of the colonies to a confir
24、mation test. 4.4 From the number of colonies confirmed, calculation of the number of Brochothrix thermosphacta per millilitre or per gram of sample from colonies obtained on plates at dilution levels chosen so as to give the most reliable result (see 9.3). 5 Dilution fluid, culture media and reagent
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