BS-EN-12353-2006.pdf
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1、BRITISH STANDARD BS EN 12353:2006 Chemical disinfectants and antiseptics Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity The European Standard EN 12353:2006 has the status of a British Standard ICS 07.100.99; 11.080.20; 71.100.35 ? Licens
2、ed Copy: sheffieldun sheffieldun, na, Sat Oct 28 05:30:24 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN 12353:2006 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2006 BSI 2006 ISBN 0 580 48517 X National foreword This British Stan
3、dard is the official English language version of EN 12353:2006. It supersedes BS EN 12353:2000 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee CH/216, Chemical disinfectants and antiseptics, which has the responsibility to: A list of organizations rep
4、resented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Inde
5、x”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself conf
6、er immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate t
7、hem in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN title page, pages 2 to 23 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. DateComments
8、Licensed Copy: sheffieldun sheffieldun, na, Sat Oct 28 05:30:24 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 12353 June 2006 ICS 07.100.99; 11.080.20; 71.100.35Supersedes EN 12353:1999 English Version Chemical disinfectants and antiseptics - Preserv
9、ation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity Antiseptiques et dsinfectants chimiques - Conservation des organismes test utiliss pour la dtermination de lactivit bactricide, mycobactricide, sporicide et fongicide Chemische Desinfektionsmittel
10、und Antiseptika - Aufbewahrung von Testorganismen fr die Prfung der bakteriziden, mykobakteriziden, sporiziden und fungiziden Wirkung This European Standard was approved by CEN on 23 February 2006. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditio
11、ns for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three off
12、icial versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria,
13、 Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STAN
14、DARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2006 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 12353:2006: E Licensed Copy: sheffieldun sheffield
15、un, na, Sat Oct 28 05:30:24 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN 12353:2006 (E) 2 Contents Page Foreword3 Introduction.4 1 Scope5 2 Normative references5 3 Terms and definitions .5 4 Requirements.6 5 Methods6 5.1 Principle6 5.2 Materials and reagents6 5.3 Apparatus and glassware.8 5.4 Proce
16、dure for preservation of test organisms General. 10 5.5 Procedure for preservation of bacteria (incl. spore-forming bacteria, excl. bacterial spores) and yeasts . 10 5.6 Procedure for preservation of mycobacteria. 11 5.7 Procedure for preservation of moulds (e.g. Aspergillus niger). 12 5.8 Verificat
17、ion of the purity and identity of test organisms 13 5.9 Documentation 13 Annex A (informative) Test organisms Culture collection references and relation to CEN/TC 216 standards and prENs 15 Annex B (informative) Graphical representations 18 Bibliography. 22 Licensed Copy: sheffieldun sheffieldun, na
18、, Sat Oct 28 05:30:24 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN 12353:2006 (E) 3 Foreword This European Standard (EN 12353:2006) has been prepared by Technical Committee CEN/TC 216 “Chemical disinfectants and antiseptics”, the secretariat of which is held by AFNOR. This European Standard shall b
19、e given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2006, and conflicting national standards shall be withdrawn at the latest by December 2006. This European Standard supersedes EN 12353:1999. It was revised to include my
20、cobacteria and spore-forming bacteria and to harmonize the structure and wording with other CEN/TC 216 standards. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus
21、, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Licensed Copy: sheffieldun sheffieldun, na,
22、 Sat Oct 28 05:30:24 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN 12353:2006 (E) 4 Introduction Standardized tests for the determination of bactericidal, mycobactericidal, sporicidal and fungicidal activity of chemical disinfectants and antiseptics necessitate the use of test organisms whose purity
23、 and identity have been verified and whose biological behaviour remains stable. Therefore it is essential to specify the storage requirements. This European Standard aims at describing methods for preservation of test organisms used for such purposes. Licensed Copy: sheffieldun sheffieldun, na, Sat
24、Oct 28 05:30:24 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN 12353:2006 (E) 5 1 Scope This European Standard specifies methods for keeping test organisms used and defined in European Standards for the determination of bactericidal, mycobactericidal, sporicidal and fungicidal activity of chemical di
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