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1、BRITISH STANDARD BS EN 1390:2006 Incorporating corrigendum no. 1 Wood preservatives Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae Laboratory method The European Standard EN 1390:2006 has the status of a British Standard ICS 71.100.50 ? Licensed Copy: London South
2、 Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) BSI BS EN 1390:2006 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2006 BSI 2007 ISBN 978 0 580 60059 3 National foreword This
3、 British Standard is the UK implementation of EN 1390:2006. It supersedes DD ENV 1390:1995 and BS 5219:1975 which are withdrawn. The UK participation in its preparation was entrusted by Technical Committee B/515, Wood preservatives, to Subcommittee B/515/1, Preservative preconditioning and biologica
4、l testing. A list of organizations represented on this subcommittee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confe
5、r immunity from legal obligations. Amendments issued since publication Amd. No. DateComments 17378 Corrigendum No. 1 31 October 2007Addition to supersession details Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) B
6、SI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 1390 June 2006 ICS 71.100.50Supersedes ENV 1390:1994 English Version Wood preservatives - Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae - Laboratory method Produits de prservation du bois - Dtermination de l
7、action curative contre les larves dHylotrupes bajulus (Linnaeus) - Mthode de laboratoire Holzschutzmittel - Bestimmung der bekmpfeden Wirkung gegenber Larven von Hylotrupes bajulus (Linnaeus) - Laboratoriumsverfharen This European Standard was approved by CEN on 24 May 2006. CEN members are bound to
8、 comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Centra
9、l Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as th
10、e official versions. CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Sloven
11、ia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2006 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN na
12、tional Members. Ref. No. EN 1390:2006: E Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) BSI EN 1390:2006 (E) 2 Contents Page Foreword3 Introduction.4 1 Scope5 2 Normative references5 3 Terms and definitions .5 4 P
13、rinciple5 5 Test materials.6 6 Sampling.7 7 Test specimens7 8 Procedure.9 9 Validity of the test. 12 10 Expression of results . 12 11 Test report. 13 Annex A (informative) Example of a test report 14 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus 16 Annex C (informative)
14、Environmental, health and safety precautions within chemical/biological laboratory 19 Bibliography. 20 Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) BSI EN 1390:2006 (E) 3 Foreword This document (EN 1390:2006) ha
15、s been prepared by Technical Committee CEN/TC 38 “Durability of wood and derived materials”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 200
16、6, and conflicting national standards shall be withdrawn at the latest by December 2006. This document supersedes ENV 1390:1994. Significant technical differences between this standard and ENV 1390:1994 are as follows: a) introduction of new harmonised specifications for the test specimens used in t
17、he diverse biological tests; b) separation of the method according to the expected test periods for fast and slow acting preservatives and for deferred acting preservatives respectively; c) admission of the terms given in EN 1001-1and the definitions of EN 1001-2; d) introduction of an informative A
18、nnex to take account of consideration for minimisation of environmental and health hazards caused by the use of this biological test. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Aust
19、ria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Licensed Copy: London S
20、outh Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) BSI EN 1390:2006 (E) 4 Introduction This document describes a laboratory method of testing which gives a basis for the assessment of the eradicant action of fast and slow acting wood preser
21、vatives and of deferred acting wood preservatives against Hylotrupes bajulus. It allows determination of the lethal effect of a surface application of a preservative product on a population of large larvae previously introduced into the test specimens. The method simulates conditions in practice whe
22、re a beam is treated, which is only slightly attacked and where cutting away has not exposed insect tunnels. This represents a severe test of the product. In some particular instances, for example where the preservative is to be used on timbers of large dimensions, laminated beams, blockboard, plywo
23、od and other panel products, other test methods can be used to obtain complementary information on the effectiveness of the eradicant action of a product. Such methods lie outside the scope of this document. This laboratory method provides one criterion by which the value of a product can be assesse
24、d. In making this assessment the methods by which the preservative may be applied should be taken into account. lt is further recommended that results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products tha
25、t are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of sepa
26、rate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel, (see also Annex C for environmental, health and safety precautions). Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontr
27、olled Copy, (c) BSI EN 1390:2006 (E) 5 1 Scope This document specifies a method for the determination of the eradicant action of a surface application of a fast and a slow acting wood preservative product or a deferred acting wood preservative product on timber infested with larvae of Hylotrupes baj
28、ulus (Linnaeus). This method is applicable to: organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or water-soluble products, for example,
29、salts. NOTE An ageing procedure cannot be combined with this method. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced do
30、cument (including any amendments) applies. EN ISO 3696,Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) ISO 835-1:1981, Laboratory glassware - Graduated pipettes - Part 1: General requirements 3 Terms and definitions For the purposes of this document, the followin
31、g terms and definitions apply. 3.1 representative sample sample with physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood pr
32、eservative to be tested) Adapted from EN 1001-2:2005, 4.83 4 Principle Insertion of larvae of Hylotrupes bajulus into test specimens. After a period of time to allow the larvae to establish themselves in the test specimens, treatment of these test specimens by brushing or pipetting of the test prese
33、rvative product. After the time necessary for the preservative to act effectively, assessment of the mortality of the larvae compared with that of larvae in untreated control test specimens. Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007
34、, Uncontrolled Copy, (c) BSI EN 1390:2006 (E) 6 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae 5.1.2 Source of larvae. The larvae shall preferably be obtained from cultures reared according to the method described in Annex B. NOTE Larvae can also be taken from na
35、turally infested wood, in which case they should be transferred into sapwood of pine and stored for at least four weeks under the rearing conditions specified in Annex B. Do not use the larvae in the test if they have not fed normally during this storage period. 5.1.3 Provision of larvae Carefully s
36、plit or crumble infested blocks to extract larvae. Keep the larvae separate from one another in glass receptacles for two or three days in the culturing chamber (5.3.1) to check they are healthy. 5.1.4 Choice of larvae Use only healthy larvae in the test. NOTE 1 A healthy larva can be recognized by
37、ivory-white colour, its firm consistency and rounded appearance, and by the absence of wounds or bites, which show up as dark marks. Healthy larvae react to the touch by vigorous movement and attempts to bite. Reject any larvae, which are shrunken or aged which have recently moulted, or which are in
38、 a pre-pupal stage. Weigh each larva and place it in a glass receptacle marking the receptacle with the weight of the larva. Make up two groups with the weight ranges: - 51 mg to 100 mg and -101 mg to 150 mg. NOTE 2 Larvae with a mass larger than 150 mg in mass are unsuitable as they can pupate duri
39、ng the course of the test. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found suitable. 5.2.2 Gelatine, for sealing the
40、 relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at
41、the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) BSI E
42、N 1390:2006 (E) 7 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (28 2) C and at a relative humidity of (70 5) %. 5.3.2 Ventilated fume cupboard, in which the specimens are treated with an input air temperature of (20 5) C and a maximum air speed, measured at the input op
43、ening with the sash in the approximate operating position, of 0,5 m/s. CAUTION - It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.3 Testing chamber, ventilated and controlled at (21 2) C an
44、d at a relative humidity of (75 5) %. 5.3.4 Drill and twist drills, with 3 mm, 4 mm and 5 mm diameters. 5.3.5 Pipettes as specified in ISO 835-1:1981, Class B - graduated pipette with no waiting time, with a capacity 5 ml and an accuracy of 0,05 ml. 5.3.6 Safety equipment and protective clothing, ap
45、propriate for the test product and the test, to ensure the safety of the operator. 5.3.7 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 1 mg. 5.3.8 Rectangular cover with sides, constructed either of glass, plastics, plywood and of a height not less than 200
46、 mm and with an open face of sufficient size to cover all the treated specimens from a single test. 5.3.9 X-ray apparatus (optional) with tungsten target and beryllium window, with voltage and current continuously variable in the ranges: -voltage: 10 kV to 50 kV; -current: 0 mA to 15 mA. 5.3.10 Prot
47、ective gloves 6 Sampling The sample of preservative shall represent the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier. NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
48、 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus)1). NOTE Additional tests may be carried out using other species but, if so, this should be stated in the test report. 1) In southern European countries the species of pine most frequently infested b
49、y Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been demonstrated in all aspects (development of larvae, resistance to impregnation, etc.). Licensed Copy: London South Bank University, London South Bank University, Wed Oct 17 01:21:17 GMT+00:00 2007, Uncontrolled Copy, (c) BSI EN 1390:2006 (E) 8 7.2 Wood quality The wood shall be free from visible cracks, stain, decay, insec
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