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1、BRITISH STANDARD BS EN 49-2: 1992 Wood preservatives Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival Part 2: Application by impregnation (laboratory method) The European Standard EN 49-2:1992 has the status of a British Standard UDC
2、 674.048.4:620.1 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN 49-2:1992 This British Standard, having been prepared under the direction of the Technical Sector Board for Building and Civil Engineering (B/-), was published under the
3、authority of the Standards Board and comes into effect on 15 November 1992 BSI 12-1999 The following BSI references relate to the work on this standard: Committee reference B/515 Draft for comment 90/50083 DC ISBN 0 580 20950 4 Cooperating organizations The European Committee for Standardization (CE
4、N), under whose supervision this European Standard was prepared, comprises the national standards organizations of the following countries: AustriaOesterreichisches Normungsinstitut BelgiumInstitut belge de normalisation DenmarkDansk Standardiseringsraad FinlandSuomen Standardisoimisliito, r.y. Fran
5、ceAssociation franaise de normalisation GermanyDeutsches Institut fr Normung e.V. GreeceHellenic Organization for Standardization IcelandTechnological Institute of Iceland IrelandNational Standards Authority of Ireland ItalyEnte Nazionale Italiano di Unificazione LuxembourgInspection du Travail et d
6、es Mines NetherlandsNederlands Normalisatie-instituut NorwayNorges Standardiseringsforbund PortugalInstituto Portugus da Qualidade SpainAsociacin Espaola de Normalizacin y Certificacin SwedenStandardiseringskommissionen i Sverige SwitzerlandAssociation suisse de normalisation United KingdomBritish S
7、tandards Institution Amendments issued since publication Amd. No.DateComments Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN 49-2:1992 BSI 12-1999i Contents Page Cooperating organizationsInside front cover National forewordii Foreword
8、2 Text of EN 49-23 National annex NA (informative) Committees responsibleInside back cover National annex NB (informative) Cross-referencesInside back cover Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN 49-2:1992 ii BSI 12-1999 Natio
9、nal foreword This Part of BS EN 49 has been prepared under the direction of the Technical Sector Board for Building and Civil Engineering and is the English language version of EN 49-2 Wood preservatives Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying
10、and larval survival Part 2: Application by (Laboratory method):1992, published by the European Committee for Standardization (CEN). EN 49-2 was produced as a result of international discussion in which the United Kingdom took an active part. BS EN 49-2 supersedes BS 5437:1977, which is withdrawn. BS
11、 EN 49 consists of the following Parts. Part 1: Application by surface treatment (laboratory method); Part 2: Application by impregnation (laboratory method). Part 1 is identical to EN 49-1:1992 The principal differences between BS 5437:1977 and this Part of BS EN 49 include an extension of the scop
12、e of the method to organic water dispersible formulations; a more detailed description of the method by which the insects may be cultured; the establishment of viscosity limits for the cellulose paste; additional information to be included in the test report. CAUTION. Attention is drawn to the Healt
13、h and Safety at Work, etc. Act 1974, and the need for ensuring that the method specified in this Part of BS EN 49 is carried out with suitable precautions. The procedure described in this Part of BS EN 49 is intended to be carried out by appropriately qualified and experienced persons or other suita
14、bly trained and or supervised personnel. Attention is drawn to the precautions given in the introduction, 5.2.5 and 5.3.4. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance w
15、ith a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, the EN title page, pages 2 to 12, an inside back cover and a back cover. This standard has been updated (see copyright date
16、) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 49-2 September 1992 UDC
17、 674.048.4:620.1Together with EN 49-1 will supersede EN 49:1976 Descriptors: Wood, wood preservatives, pesticides, insecticides, pest control, laboratory tests, effectiveness, prevention, anobiidae English version Wood preservatives Determination of the protective effectiveness against Anobium punct
18、atum (De Geer) by egg-laying and larval survival Part 2: Application by impregnation (Laboratory method) Produits de prservation du bois Dtermination de lefficacit protectrice vis-vis Anobium punctatum (De Geer) par lobservation de la ponte et du taux de survie des larves Partie 2: Application par t
19、raitement et profondeur (Mthode de laboratoire) Holzschutzmittel Bestimmung der vorbeugenden Wirkung gegenber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des berlebens von Larven Teil 2: Anwendung durch Volltrnkung (Laboratoriumsverfahren) This European Standard was approved by CEN
20、 on 1992-09-25. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may
21、be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Centr
22、al Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. CEN European C
23、ommittee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1992 Copyright reserved to CEN members Ref No. EN 49-2:1992 E Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontroll
24、ed Copy, (c) BSI EN 49-2:1992 BSI 12-1999 2 Foreword This Part of this European Standard has been drawn up by the “Anobium” Expert Group of CEN/TC 38 “Durability of wood and wood-based products” with AFNOR as secretariat. This Part of EN 49 together with EN 49-1 replaces EN 49:1976. This Part of EN
25、49 is required to enable effectiveness assessments of preservatives which are intended to be applied by impregnation. This Part of this European Standard shall be given the status of a national standard, either by publication of an identical test or by endorsement, at the latest by March 1993, and c
26、onflicting national standards shall be withdrawn at the latest by March 1993. This Part of this European Standard was adopted by CEN and in accordance with the Common CEN/CENELEC Rules, the following countries are bound to implement this Part of this European Standard: Austria, Belgium, Denmark, Fin
27、land, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. Contents Page Foreword2 Introduction3 1Scope3 2Normative references3 3Definitions3 4Principle3 5Test materials and apparatus4 6Sampling4 7Test specimens5
28、8Procedure5 9Validity of test7 10Expression of results7 11Test report7 Annex A (informative) Example of a test report9 Annex B (informative) Identification of sex of test insects (Anobium punctatum)10 Annex C (informative) Culturing technique for Anobium punctatum10 Annex D (informative) Bibliograph
29、y12 Figure B.1 Last segment of the abdomen of Anobium punctatum for the identification of sex11 Table A.1 Results9 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN 49-2:1992 BSI 12-19993 Introduction This Part of this European Standard des
30、cribes a laboratory method of test which gives a basis for assessment of the effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the concentration at which the product prevents the development of infestation from egg laying. The results of this test comple
31、ment those derived from testing by the larval transfer method (see EN 21). The method simulates conditions which can occur in practice on timber which has been treated some time previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum are laid. This laboratory me
32、thod provides one criterion by which the value of a product can be assessed. In making this assessment the methods by which the preservative may be applied should be taken into account. It is further recommended that results from this test should be supplemented by those from other appropriate tests
33、, and above all by comparison with practical experience. When products which are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory
34、apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel. 1 Scope This Part of EN 49 specifies a method for the determination of the protective effectiveness or the toxic
35、 values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in wood which has been treated previously by full impregnation. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides or; organic formulations, as sup
36、plied or as prepared in the laboratory by dilution of concentrates; or organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; or water-soluble materials, for example salts. NOTEThis method may be used in conjunction with an ageing procedure,
37、for example EN 73. 2 Normative reference This European Standard incorporates by dated or undated reference, provisions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, subsequent amend
38、ments to or revisions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies. ISO 3696:1987, Water for analytical laboratory use Specification and test method
39、s. 3 Definitions For the purposes of this Part of EN 49, the following definitions apply. 3.1 representative sample a sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled 3.2 supplier the sponsor of the test 4 Pr
40、inciple Depending on the test being carried out either a set of test specimens of a susceptible wood species is impregnated with a solution of the preservative; or if toxic values are to be determined, several sets of test specimens of a susceptible wood species are impregnated with a series of solu
41、tions in which the concentration of preservative is ranged in a given progression. The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs laid, the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in untreated contro
42、ls. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated controls. Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 04 09:00:14 GMT+00:00 2006, Uncontrolled Co
43、py, (c) BSI EN 49-2:1992 4 BSI 12-1999 5 Test materials and apparatus 5.1 Biological material 5.1.1 Anobium punctatum (De Geer) Adult males and females in good condition. Adults to be used in the test shall be collected daily from naturally infested wood or laboratory culture (see Annex C). Use rece
44、ntly emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and then checked to ensure that they are undamaged, active, and free from any infestation by mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and females in s
45、eparate containers. NOTEThe proportion of males and females varies during the emergence period. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the end sections of test specimens. NOTEParaffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Paste, for securing
46、 filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and insoluble in the product under test. NOTESodium carboxymethyl cellulose, food grade, has been found to be suitable. 5.2.3 Xylene, technical grade, mixed isomers. 5.2.4 Water, complying with grade 3 of ISO 3696. 5.2.5 S
47、olvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION. Do not use benzene or other solvents which pose a health risk. 5.2.6 Filte
48、r paper, ordinary quality, medium-fast grade. 5.2.7 Fine cloth, of cotton or linen, with a mesh aperture of 0,3 mm to 0,6 mm. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (21 1) C, and at relative humidity (80 5) %. 5.3.2 Conditioning chamber, well ventilated, controlle
49、d at (20 2) C and relative humidity (65 5) %. NOTEThe conditioning of specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Treatment vessels, of a material that does not react with the preservative under test, for example of glass for organic products and of polyethylene for salts containing fluorine. 5.3.4 Laboratory work area, well ventilated, where treatment of the test specimen
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