BS-EN-ISO-20645-2004.pdf
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1、BRITISH STANDARD BS EN ISO 20645:2004 Textile fabrics Determination of antibacterial activity Agar diffusion plate test The European Standard EN ISO 20645:2004 has the status of a British Standard ICS 59.080.30 ? Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 13:36:46 GMT+00:00 2006, Uncontr
2、olled Copy, (c) BSI BS EN ISO 20645:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 20 December 2004 BSI 20 December 2004 ISBN 0 580 45126 7 National foreword This British Standard is the official English language version of EN ISO 20645
3、:2004. It is identical with ISO 20645:2004. The UK participation in its preparation was entrusted to Technical Committee TCI/80, Textiles Chemical testing, which has the responsibility to: A list of organizations represented on this committee can be obtained on request to its secretary. Cross-refere
4、nces The British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British
5、Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to
6、 the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside
7、 front cover, the EN ISO title page, pages 2 to 13 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. DateComments Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 13:36:46 GMT+00:00 20
8、06, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 20645 December 2004 ICS 59.080.30 English version Textile fabrics - Determination of antibacterial activity - Agar diffusion plate test (ISO 20645:2004) Etoffes - Contrle de lactivit antibactrienne - Essai de dif
9、fusion sur plaques de glose (ISO 20645:2004) Textile Flchengebilde - Prfung der antibakteriellen Wirkung - Agarplattendiffusionstest (ISO 20645:2004) This European Standard was approved by CEN on 2 September 2004. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipul
10、ate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exi
11、sts in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bo
12、dies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE F
13、OR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2004 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 20645:2004: E Licensed Copy: sheffieldu
14、n sheffieldun, na, Sun Nov 19 13:36:46 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 20645:2004 (E) 2 Contents Page Foreword3 Introduction.4 1 Scope 5 2 Terms and definitions .5 3 Safety precautions.5 4 Principle5 5 Apparatus, reagents and culture media5 5.1 Apparatus .5 5.2 Reagents and culture
15、 media.6 6 Test bacteria.7 7 Preparation of the bacteria cultures7 7.1 General7 7.2 Culturing with lyophilized bacteria7 7.3 Culturing from the agar.7 8 Preparation of test specimens .8 8.1 General8 8.2 Preparation of the test material8 8.3 Material for blind tests 8 8.4 Conditioning of specimens.8
16、9 Test procedure.8 10 Assessment of the tests .9 11 Evaluation.10 12 Test report10 Annex A (informative) Special specimens and tests.11 Annex B (informative) European Suppliers of ATCC.12 Bibliography13 Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 13:36:46 GMT+00:00 2006, Uncontrolled Copy
17、, (c) BSI EN ISO 20645:2004 (E) 3 Foreword This document (EN ISO 20645:2004) has been prepared by Technical Committee CEN/TC 248 “Textiles and textile products”, the secretariat of which is held by BSI, in collaboration with Technical Committee ISO/TC 38 “Textiles”. This European Standard shall be g
18、iven the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2005, and conflicting national standards shall be withdrawn at the latest by June 2005. This document includes a Bibliography. CAUTION This method involves the use of processes
19、 that could lead to a hazardous situation. Attention is drawn to the safety precautions in Clause 3. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Repub
20、lic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 13:36:46 G
21、MT+00:00 2006, Uncontrolled Copy, (c) BSI EN ISO 20645:2004 (E) 4 Introduction The application of an antimicrobial finish to a textile can prevent bacterial growth and might reduce the effects of microbial pathway products, biodeterioration and microbiogenous odours. This method determines the activ
22、ity of such treatments qualitatively when different products are compared. Semi-quantitative information on the effect of treatments can be obtained when different concentrations of the same product are compared. Licensed Copy: sheffieldun sheffieldun, na, Sun Nov 19 13:36:46 GMT+00:00 2006, Uncontr
23、olled Copy, (c) BSI EN ISO 20645:2004 (E) 5 1 Scope This document specifies a method for the determination of the effect of antibacterial treatments applied to woven, knitted and other flat textiles. This method is applicable to testing hygienic finishes of hydrophilic, air-permeable materials or an
24、tibacterial products incorporated in the fibre. A minimum diffusion of the antibacterial treatment into the test agar is necessary with this procedure. NOTE Other materials may be tested using this method, provided that it is adapted accordingly. This method is not suitable for testing textiles trea
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