BS-EN-ISO-8199-2007.pdf
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1、BRITISH STANDARD BS EN ISO 8199:2007 Incorporating amendment no. 1 (renumbers BS ISO 8199:2005 as BS EN ISO 8199:2007) Water quality General guidance on the enumeration of micro-organisms by culture The European Standard EN ISO 8199:2007 has the status of a British Standard ICS 07.100.20 ? BS EN ISO
2、 8199:2007 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 5 October 2005 BSI 2008 ISBN 978 0 580 59549 3 National foreword This British Standard is the UK implementation of EN ISO 8199:2007. It supersedes BS ISO 8199:2005 which is withdrawn.
3、 The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/4, Biological methods. A list of organizations represented on this subcommittee can be obtained on request to its secretary. This publication does not purport to include all the ne
4、cessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunity from legal obligations. Amendments/corrigenda issued since publication DateComments 31 January 2008Renumbers BS ISO 8199:2005 as BS EN ISO 8199:2007 EUROPEAN
5、 STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 8199 October 2007 ICS 07.100.20 English Version Water quality - General guidance on the enumeration of micro- organisms by culture (ISO 8199:2005) Qualit de leau - Lignes directrices gnrales pour le dnombrement des micro-organismes sur milieu de cultu
6、re (ISO 8199:2005) Wasserbeschaffenheit - Allgemeine Anleitung zur Zhlung von Mikroorganismen durch Kulturverfahren (ISO 8199:2005) This European Standard was approved by CEN on 27 September 2007. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the condition
7、s for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three of
8、ficial versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austr
9、ia, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMI
10、TTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2007 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 8199:2007: E Foreword The text of
11、 ISO 8199:2005 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 8199:2007 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standar
12、d shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by April 2008, and conflicting national standards shall be withdrawn at the latest by April 2008. According to the CEN/CENELEC Internal Regulations, the national standards
13、organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portuga
14、l, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 8199:2005 has been approved by CEN as a EN ISO 8199:2007 without any modification. EN ISO 8199:2007 Reference number ISO 8199:2005(E) INTERNATIONAL STANDARD ISO 8199 Second edition 2
15、005-06-15 Water quality General guidance on the enumeration of micro-organisms by culture Qualit de leau Lignes directrices gnrales pour le dnombrement des micro-organismes sur milieu de culture EN ISO 8199:2007 ii iii Contents Page Foreword iv Introduction v 1 Scope . 1 2 Normative references . 1 3
16、 Principle. 1 4 General. 1 5 Diluents and culture media 2 6 Sterilization of apparatus and glassware . 5 7 Samples . 5 8 Enumeration after inoculation of test portions of the sample in (or on) solid media . 6 9 Enumeration by inoculation in liquid media 15 Annex A (informative) Criteria for the choi
17、ce of enumeration technique. 22 Annex B (informative) MPN tables 27 Bibliography . 37 EN ISO 8199:2007 iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is nor
18、mally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in
19、the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepar
20、e International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that som
21、e of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 8199 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4, Microbiological methods. This second edition cancels and
22、 replaces the first edition (ISO 8199:1988), which has been technically revised. EN ISO 8199:2007 v Introduction Techniques for the isolation and enumeration of micro-organisms, based on their ability to grow on specified culture media, are an important and widely used means of assessing the microbi
23、ological quality of water. The purpose of this guide is to gather in a single document the information common to the various enumeration techniques so as to avoid repetition of technical details in individual standards and to facilitate the choice of the technique most suitable for a particular prob
24、lem. EN ISO 8199:2007 blank 1 Water quality General guidance on the enumeration of micro- organisms by culture WARNING Persons using this International Standard should be familiar with normal laboratory practice. This standard does not purport to address all of the safety problems, if any, associate
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