BS-EN-ISO-10253-2006.pdf
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1、BRITISH STANDARD BS EN ISO 10253:2006 Water quality Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum The European Standard EN ISO 10253:2006 has the status of a British Standard ICS 13.060.70 ? Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 01:51:35
2、 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS EN ISO 10253:2006 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 28 April 2006 BSI 2006 ISBN 0 580 48246 4 National foreword This British Standard is the official English language version of EN
3、ISO 10253:2006. It is identical with ISO 10253:2006. It supersedes BS EN ISO 10253:1998 which is withdrawn. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/5, Biological methods, which has the responsibility to: A list of organiz
4、ations represented on this subcommittee can be obtained on request to its secretary. Cross-references The British Standards which implement international or European publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Corres
5、pondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not o
6、f itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and
7、 promulgate them in the UK. Summary of pages This document comprises a front cover, an inside front cover, the EN ISO title page, the EN ISO foreword page, the ISO title page, pages ii to iv, pages 1 to 12, an inside back cover and a back cover. The BSI copyright notice displayed in this document in
8、dicates when the document was last issued. Amendments issued since publication Amd. No. DateComments Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 01:51:35 GMT+00:00 2006, Uncontrolled Copy, (c) BSI EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10253 April 2006 ICS 13.060.70Super
9、sedes EN ISO 10253:1998 English Version Water quality - Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum (ISO 10253:2006) Qualit de leau - Essai dinhibition de la croissance des algues marines avec Skeletonema costatum et Phaeodactylum tricornutum (ISO 1025
10、3:2006) Wasserbeschaffenheit - Wachstumshemmtest mit marinen Algen (kursiv)Skeletonema costatum und (kursiv)Phaeodactylum tricornutum (ISO 10253:2006) This European Standard was approved by CEN on 13 April 2006. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulat
11、e the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exist
12、s in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards bodi
13、es of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMM
14、ITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2006 CENAll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10253:2006: E Licensed Copy: she
15、ffieldun sheffieldun, na, Sat Nov 18 01:51:35 GMT+00:00 2006, Uncontrolled Copy, (c) BSI Foreword This document (EN ISO 10253:2006) has been prepared by Technical Committee ISO/TC 147 “Water quality“ in collaboration with Technical Committee CEN/TC 230 “Water analysis“, the secretariat of which is h
16、eld by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2006, and conflicting national standards shall be withdrawn at the latest by October 2006. This document supersedes EN ISO 10253
17、:1998. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Lat
18、via, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 10253:2006 has been approved by CEN as EN ISO 10253:2006 without any modifications. EN ISO 10253:2006 Licensed Copy
19、: sheffieldun sheffieldun, na, Sat Nov 18 01:51:35 GMT+00:00 2006, Uncontrolled Copy, (c) BSI Reference number ISO 10253:2006(E) INTERNATIONAL STANDARD ISO 10253 Second edition 2006-04-15 Water quality Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum Qualit
20、 de leau Essai dinhibition de la croissance des algues marines avec Skeletonema costatum et Phaeodactylum tricornutum EN ISO 10253:2006 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 01:51:35 GMT+00:00 2006, Uncontrolled Copy, (c) BSI ii Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18
21、 01:51:35 GMT+00:00 2006, Uncontrolled Copy, (c) BSI iii Contents Page Foreword iv 1 Scope . 1 2 Normative references. 1 3 Terms and definitions. 1 4 Principle. 2 5 Materials 2 5.1 Test organisms . 2 5.2 Water 3 5.3 Sea water . 3 5.4 Nutrients 4 6 Apparatus 5 7 Procedure 5 7.1 Preparation of growth
22、medium 5 7.2 Preparation of pre-culture and inoculum. 5 7.3 Choice of test concentrations . 6 7.4 Preparation of test substance stock solutions 6 7.5 Preparation of test and control batches. 6 7.6 Incubation 7 7.7 Measurements. 7 8 Validity criteria 7 9 Interpretation of data 8 9.1 Plotting growth c
23、urves. 8 9.2 Calculation of percentage inhibition. 8 9.3 Determination of EC(r)x 9 10 Expression of results . 9 11 Interpretation of results 9 12 Reproducibility 9 13 Test report . 10 Annex A (informative) Preparation of dilution series of mixtures in sea water (effluents or elutriates) 11 Bibliogra
24、phy. 12 EN ISO 10253:2006 Licensed Copy: sheffieldun sheffieldun, na, Sat Nov 18 01:51:35 GMT+00:00 2006, Uncontrolled Copy, (c) BSI iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing
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