BS-6057-3.23-1990 ISO-9252-1989.pdf
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1、BRITISH STANDARD BS 6057-3.23: 1990 ISO 9252:1989 Rubber latices Part 3: Methods of test Section 3.23 Microbiological examination of synthetic rubber latex NOTEAttention is drawn to BS 6057-0 “General introduction”, issued separately. Licensed Copy: sheffieldun sheffieldun, na, Wed Dec 06 15:21:05 G
2、MT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6057-3.23:1990 This British Standard, having been prepared under the direction of the Rubber Standards Policy Committee, was published under the authority of the Board of BSI and comes into effect on 30 September 1990 BSI 11-1999 The following BSI referen
3、ces relate to the work on this standard: Committee reference RUM/38 Draft for comment 88/37362 DC ISBN 0 580 18867 1 Committees responsible for this British Standard The preparation of this British Standard was entrusted by the Rubber Standards Policy Committee (RUM/-) to Technical Committee RUM/38,
4、 upon which the following bodies were represented: British Rubber Manufacturers Association Malaysian Rubber Producers Research Association Medical Sterile Products Association Tropical Growers Association Amendments issued since publication Amd. No.DateComments Licensed Copy: sheffieldun sheffieldu
5、n, na, Wed Dec 06 15:21:05 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6057-3.23:1990 BSI 11-1999i Contents Page Committees responsibleInside front cover National forewordii 1Scope1 2Normative references1 3Definition1 4Principle1 5Reagents1 6Apparatus1 7Sampling1 8Selection of media2 9Procedure2 1
6、0Expression of results3 11Precision3 12Test report3 Annex A (informative) Preparation of media and plates4 Publication(s) referred toInside back cover Licensed Copy: sheffieldun sheffieldun, na, Wed Dec 06 15:21:05 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6057-3.23:1990 ii BSI 11-1999 National
7、foreword This Section of BS 6057 has been prepared under the direction of the Rubber Standards Policy Committee and is identical with ISO 9252:1989 “Synthetic rubber latex Microbiological examination”, published by the International Organization for Standardization (ISO). Annex A of this standard is
8、 for information only. The introduction to ISO 9252 reads as follows. Synthetic latices are susceptible to post-production contamination with micro-organisms during storage and shipment. Unless precautions are taken such as maintenance of a high pH, the addition of biocide and inspection and cleanin
9、g of tanks, these organisms may proliferate, ultimately producing unpleasant odours and changes in the chemical and physical properties of the latex. It is highly desirable to be able to detect the presence of significant micro-organisms before such changes develop. The method described can be carri
10、ed out by any reasonably competent general testing laboratory, provided the operator has been trained in basic microbiological techniques. Alternatively, the method would be routine for a specialist microbiological laboratory. A British Standard does not purport to include all the necessary provisio
11、ns of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Cross-references International StandardCorresponding British Standard ISO 123:1985 BS 6057 Rubber latices Part 2:1
12、987 Sampling (Identical) ISO 976:1986Section 3.9:1990 Determination of pH (Identical) ISO 4833:1978 BS 5763 Methods for microbiological examination of food and animal feeding stuffs Part 1:1979 Enumeration of micro-organisms colony count technique at 30 C (poured plate technique) (Identical) ISO 688
13、7:1983Part 6:1983 Preparation of dilutions (Identical) ISO 7218:1985Part 0:1986 General laboratory practices (Identical) Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages 1 to 6, an inside back cover and a back cover. This standard has been updated
14、(see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Licensed Copy: sheffieldun sheffieldun, na, Wed Dec 06 15:21:05 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6057-3.23:1990 BSI 11-19991 1 Scope This International
15、 Standard specifies a method for the microbiological examination of synthetic rubber latices for the presence and approximate concentration of viable aerobic and facultative anaerobic micro-organisms. Identification of the micro-organisms is outside the scope of this International Standard, though a
16、 skilled microbiologist will be able to derive some information on them from recovered cultures. 2 Normative references The following standards contain provisions which through reference in this text, constitute provisions of this International Standard. At the time of publication, the editions indi
17、cated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid In
18、ternational Standards. ISO 123:1985, Rubber latex Sampling. ISO 976:1986, Rubber latices Determination of pH. ISO 4833:1978, Microbiology General guidance for enumeration of micro-organisms Colony count technique at 30 C. ISO 6887:1983, Microbiology General guidance for the preparation of dilutions
19、for microbiological examination. ISO 7218:1985, Microbiology General guidance for microbiological examinations. 3 Definition For the purposes of this International Standard, the following definition applies. colony a group of microbial cells derived, ideally, by the multiplication of a single organi
20、sm 4 Principle A nutrient medium is selected on the basis of the pH of the latex. The test portion of the latex is serially diluted and aliquots of each dilution are spread over the surface of a solid medium in a Petri dish, followed by incubation at 30 C for 3 days. The number of colonies of micro-
21、organisms that develop are counted, multiplied by the dilution factor and recorded as colony-forming units per cubic centimetre of the original sample. 5 Reagents Unless otherwise stated, use only distilled water or water of equivalent purity. NOTE 1Distilled water is not necessarily sterile. 5.1 Et
22、hanol. 70 % (m/m) aqueous solution (commonly called industrial grade). 5.2 Dilution fluids 5.2.1 Sodium chloride/peptone solution (see A.2.1). 5.2.2 Quarter-strength Ringer solution (see A.2.2). 5.3 Media 5.3.1 Plate count agar (see A.4.1). 5.3.2 Sabouraud dextrose agar (see A.4.2). 5.3.3 Beef extra
23、ct agar (see A.4.3). 6 Apparatus 6.1 Steam autoclave, capable of maintaining a temperature of 121 C 1 C, as described in ISO 7218. 6.2 Incubator, capable of maintaining a temperature of 30 C 1 C. 6.3 Sterile pipettes, of capacity 1 cm3 and 0,1 cm3. These may be pre-sterilized disposable pipettes or,
24、 if glass pipettes are used, they shall be clean and sterilized before use. 6.4 Petri dishes These may be obtained ready-prepared from laboratory supply houses. They may also be prepared and sterilized in the testing laboratory (see informative Annex A), provided that it is equipped to do so. 6.5 Il
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