BS-6068-5.17-1995 ISO-10229-1994.pdf
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1、BRITISH STANDARD BS 6068-5.17: 1995 ISO 10229: 1994 Water quality Part 5: Biological methods Section 5.17 Determination of the prolonged toxicity of substances to freshwater fish Method for evaluating the effects of substances on the growth rate of rainbow trout Oncorhynchus mykiss Walbaum (Teleoste
2、i, Salmonidae) Licensed Copy: sheffieldun sheffieldun, na, Tue Dec 05 01:30:41 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6068-5.17:1995 This British Standard, having been prepared under the direction of the Health and Environment Sector Board, was published under the authority of the Standards B
3、oard and comes into effect on 15 April 1995 BSI 07-1999 The following BSI references relate to the work on this standard: Committee reference EPC/44/5 Draft for comment 93/500175 DC ISBN 0 580 24131 9 Committees responsible for this British Standard The preparation of this British Standard was entru
4、sted by Technical Committee EPC/44, Water quality, to Subcommittee EPC/44/5, Biological methods, upon which the following bodies were represented: British Laboratory Ware Association Ltd. Department of the Environment (Water Directorate) Department of the Environment for Northern Ireland Department
5、of Health Freshwater Biological Association Institution of Water and Environmental Management Ministry of Agriculture, Fisheries and Food National Rivers Authority Royal Society of Chemistry Scottish Natural Heritage Soap and Detergent Industry Association Water Companies Association Water Research
6、Centre Water Services Association of England and Wales Amendments issued since publication Amd. No.DateComments Licensed Copy: sheffieldun sheffieldun, na, Tue Dec 05 01:30:41 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6068-5.17:1995 BSI 07-1999i Contents Page Committees responsibleInside front c
7、over National forewordii 1Scope1 2Normative references1 3Principle1 4Test organism2 5Reagents2 6Apparatus3 7Test environment3 8Procedure3 9Precision of the test procedure5 10Expression of results5 11Test report5 Annex A (informative) Two examples of report forms8 Annex B (informative) Bibliography11
8、 Figure 1 Examples of marks used for freeze-branding fish4 Figure 2 Responses of fish to 3, 4-dichloroaniline7 List of referencesInside back cover Licensed Copy: sheffieldun sheffieldun, na, Tue Dec 05 01:30:41 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 6068-5.17:1995 ii BSI 07-1999 National fore
9、word This Section of BS 6068 has been prepared by Subcommittee EPC/44/5 and is identical with ISO 10229:1994 Water quality Determination of the prolonged toxicity of substances to freshwater fish Method for evaluating the effects of substances on the growth rate of rainbow trout Oncorhynchus mykiss
10、Walbaum (Teleostei, Salmonidae). The international standard was prepared by Subcommittee 5, Biological methods, of Technical Committee 147, Water quality, of the International Organization for Standardization (ISO) with the active participation and approval of the UK. BS 6068 is being published in a
11、 series of Parts subdivided into Sections that will generally correspond to particular international standards. Sections are being, or will be, published in Parts 1 to 7, which, together with Part 0, are listed below. Part 0: Introduction; Part 1: Glossary; Part 2: Physical, chemical and biochemical
12、 methods; Part 3: Radiological methods; Part 4: Microbiological methods; Part 5: Biological methods; Part 6: Sampling; Part 7: Precision and accuracy. NOTEThe tests described in this Section of BS 6068 should only be carried out by suitably qualified persons with an appropriate level of biological e
13、xpertise. Usual biological procedures should be followed throughout. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immun
14、ity from legal obligations. Cross-references International standardCorresponding British Standard ISO 7346-2:1984aBS 6068-5.3:1985 Determination of the acute lethal toxicity of substances to a freshwater fish Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae): Semi-static method (Identical)
15、 ISO 7346-3:1984aSection 5.4:1985 Determination of the acute lethal toxicity of substances to a freshwater fish Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae): flow-through method (Identical) a Revision to be published shortly. Summary of pages This document comprises a front cover, an
16、inside front cover, pages i and ii, pages 1 to 12, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Licensed Copy: sheffieldun sheffieldun, na,
17、 Tue Dec 05 01:30:41 GMT+00:00 2006, Uncontrolled Copy, (c) BSI ISO 10229:1994(E) BSI 07-19991 1 Scope This International Standard specifies a method for the determination of the long-term sublethal toxicity of substances soluble in water under specified conditions to a species of anadromous fish On
18、corhynchus mykiss Walbaum (Teleostei, Salmonidae) common name, rainbow trout in fresh water. NOTE 1“Substances” is considered to include pure chemicals tested singly or in mixtures, waste waters, process waters or other waters whose sublethal toxicity to fish it is necessary to determine. A range of
19、 concentrations of waste and process waters should be tested (see 8.2). The response measured is the change in rate of growth of fish exposed to the test substance over periods of 14 d and 28 d (see 1 in Annex B). NOTE 2The mass of each fish at the start of the test, the period of exposure, the feed
20、ing regime and temperature range have been chosen to ensure that the control fish will be growing exponentially throughout the test. The method is applicable for assigning, for each test substance, a category of prolonged sublethal toxicity to Oncorhynchus mykiss under the test conditions. The resul
21、ts are insufficient by themselves to define water quality limits for environmental protection. It is possible for the method to be adapted for use with other freshwater fish and marine and brackish water fish with appropriate modification of the test conditions, particularly with respect to the temp
22、erature, the quantity and quality of the dilution water, the food and the fish-marking technique. 2 Normative references The following standards contain provisions which, through reference in this text, constitute provisions of this International Standard. At the time of publication, the editions in
23、dicated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid
24、International Standards. ISO 7346-2:1984, Water quality Determination of the acute lethal toxicity of substances to a freshwater fish Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae) Part 2: Semi-static method. ISO 7346-3:1984, Water quality Determination of the acute lethal toxicity of s
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