BS-5763-9-1986 ISO-7937-1985.pdf
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1、BRITISH STANDARD BS 5763-9: 1986 ISO 7937:1985 Methods for Microbiological examination of food and animal feeding stuffs Part 9: Enumeration of Clostridium perfringens ISO title: Microbiology General guidance for enumeration of Clostridium perfringens Colony count technique UDC 614.31 636.08 + 664:5
2、79.672:579.852.13 Licensed Copy: sheffieldun sheffieldun, na, Fri Dec 01 13:52:22 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5763-9:1986 This British Standard, having been prepared under the direction of the Food and Agriculture Standards Committee, was published under the authority of the Board
3、of BSI and comes into effect on 28 February 1986. BSI 02-1999 The following BSI references relate to the work on this standard: Committee reference FAC/21 Draft for comment 84/50323 DC ISBN 0 580 15043 7 Committees responsible for this British Standard The preparation of this British Standard was en
4、trusted by the Food and Agriculture Standards Committee (FAC/-) to Technical Committee FAC/21, upon which the following bodies were represented: Agricultural and Food Research Council: Food Research Institute British Food Manufacturing Industries Research Association British Poultry Federation Limit
5、ed Campden Food Preservation Research Association Department of Agriculture, Northern Ireland Department of Health and Social Security Department of Trade and Industry (Laboratory of the Government Chemist) Flour Milling and Baking Research Association Food Manufacturers Federation Incorporated Frui
6、t and Vegetable Canners Association Joint Committee of the Milk Marketing Board and the Dairy Trade Federation Ministry of Agriculture, Fisheries and Food Overseas Development Administration: Tropical Development and Research Institute Pet Food Manufacturers Association Public Health Laboratory Serv
7、ice Society for Applied Bacteriology UK Association of Frozen Food Producers United Kingdom Dairy Association University of Reading Amendments issued since publication Amd. No.Date of issueComments Licensed Copy: sheffieldun sheffieldun, na, Fri Dec 01 13:52:22 GMT+00:00 2006, Uncontrolled Copy, (c)
8、 BSI BS 5763-9:1986 BSI 02-1999i Contents Page Committees responsibleInside front cover National forewordii 0Introduction1 1Scope and field of application1 2Reference1 3Definitions1 4Principle1 5Diluent, culture media and reagents2 6Apparatus and glassware3 7Sampling4 8Preparation of the test sample
9、4 9Procedure4 10Expression of results5 11Test report6 Annex A Confidence intervals for estimated counts7 Table7 Publication referred toInside back cover Licensed Copy: sheffieldun sheffieldun, na, Fri Dec 01 13:52:22 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5763-9:1986 ii BSI 02-1999 National f
10、oreword This Part of BS 5763 has been prepared under the direction of the Food and Agriculture Standards Committee. It is identical with ISO 7937-1985 “Microbiology General guidance for enumeration of Clostridium perfringens Colony count technique” published by the International Organization for Sta
11、ndardization (ISO) and in the preparation of which the United Kingdom played a full part. Terminology and conventions. The text of the international standard has been approved as suitable for publication as a British Standard without deviation. Some terminology and certain conventions are not identi
12、cal with those used in British Standards; attention is drawn especially to the following. The comma has been used as a decimal marker. In British Standards it is current practice to use a full point on the baseline as the decimal marker. It is also current practice in British Standards to use the sy
13、mbol “L” for litre (and its submultiples) instead of “I” and to use the spelling “sulphur” etc. instead of “sulfur” etc. Wherever the words “International Standard” appear, referring to this standard, they should be read as “British Standard”. A British Standard does not purport to include all the n
14、ecessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Cross-reference International standardCorresponding British Standard ISO 6887-1983BS 5763 Methods
15、 of microbiological examination of food and animal feeding stuffs Part 6:1983 Preparation of dilutions (Identical) Summary of pages This document comprises a front cover, an inside front cover, page i and ii, pages 1 to 8, an inside back cover and a back cover. This standard has been updated (see co
16、pyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Licensed Copy: sheffieldun sheffieldun, na, Fri Dec 01 13:52:22 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5763-9:1986 BSI 02-19991 0 Introduction 0.1 This Internationa
17、l Standard is intended to provide general guidance for the examination of products not dealt with by existing International Standards and for the reference of bodies preparing microbiological methods of test for application to food products or to animal feeding stuffs. In view of the large variety o
18、f products within this field of application, these guidelines may not be appropriate for some products in every detail, and for some other products it may be necessary to use different methods. Nevertheless, it is hoped that, in all cases, every attempt will be made to apply these guidelines as far
19、as possible and that deviations from them will only be made if absolutely necessary for technical reasons. When this International Standard is next reviewed, account will be taken of all information then available regarding the extent to which the guidelines have been followed and the reasons which
20、necessitated deviation from them in the case of particular products. The harmonization of test methods cannot be immediate, and for certain groups of products, International Standards and/or national standards that do not comply with the guidelines may already exist. In cases where International Sta
21、ndards already exist for the product to be tested, they should be followed, but it is hoped that, when they are reviewed, they will be aligned with this International Standard so that, eventually, the only remaining departures from these guidelines will be those necessary for well established techni
22、cal reasons. 0.2 For practical reasons, the definition of Clostridium perfringens given in clause 3 and used as the basis for the procedure does not exclusively describe strains of Clostridium perfringens. In particular, the confirmatory tests are inadequate to distinguish between Clostridium perfri
23、ngens and other closely related but less commonly encountered Clostridium species such as C. paraperfringens and C. absonum. 0.3 For statistical reasons, the lowest limit for the number of colonies counted per dish has been set at 15, but, for practical purposes, a count of lower numbers of Clostrid
24、ium perfringens is often required. The confidence limits of such determinations (estimated counts) are given in the annex. 1 Scope and field of application This International Standard gives general guidance for the enumeration of viable Clostridium perfringens in products intended for human consumpt
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