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1、INTERNATIONAL STANDARD First edition 1993-07-0 1 Wheat flour and durum wheat semolina - Determination of impurities of animal origin Farines de ble tendre et semoules de bM dur- - impuret b) whole insects (larva, nymph or adult); Cl insect frag ments (inclu ding butterfly scales), sect eggs, whole m
2、ites and their frag ments. in- Count the number of impurities of size greater than 30 Pm in each category and, if required, determine the minimum size of the impurities in each category by using the micrometer eyepiece. If required, the impurities grouped together in category c) may be quantified se
3、parately. lt may be necessary to use a magnification of x 75 or x 80 to study impurities which are difficult to identify. lt may be useful to use a mounted needle (5.12) to probe the various organic substances present on the filter or to move them into a clean area in the centre of the filter. Note
4、also the presence and nature of any impurities which are not of human or animal origin and of any impurities of human or animal origin but which are not specified in the categories a) to c) above. Give a detailed description of such impurities for specifi- cation in the test report (for example, col
5、oured threads of a synthetic material, metallic debris, mineral particles, human hairs, tat fur, bird feathers or down, etc.). 4 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/21/2007 0
6、8:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 11050:1993(E) 7.6 Number of determinations Carry out two determinations on tes from the Same Iaboratory Sample. t portions taken the Same Operator using the Same equipment within a short interval of time, shall not
7、 be greater than IO fragments. 10 Test report 8 Expression of results (See the example given in annex B.) Provided that the repeatability requirement (clause 9) is satisfied, express the results separ- ately for each filter as the number of impurities found in each category. If the repeatability req
8、uirement is not satisfied, carry out two new determinations after homogenization of the laboratory Sample. If at least one rodent hair or fragment of rodent hair is found in one of the test portions, carry out four new determinations and report the results separ- ately for each of the six determinat
9、ions. 9 Repeatability The absolute differente between two independent Single test results, obtained using the Same method on identical test material in the Same laboratory by The test report shall specify - the method of s ampling (f i known) and whether the special requ irements of clause 6 we re m
10、et, - the method used, - the test resuIt(s) obtained, and - if the repeatabili ty has been ehe quote d result obt ained. cked , the final lt shall also mention all operating details not speci- fied in this International Standard, or regarded as optional, together with details of any incidents which
11、may have influenced the results. The test re Port shall include all in format ion necess- arY for the com plete identification of the Sample. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale,
12、 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 11050:1993(E) Annex A (informative) Definitions and characteristics of fragments found on the filters A.l Definitions For the purposes of this annex, the following defi- nitions apply. A.1.1 abdomen: R
13、ear part of the body of an insect, excluding head and thorax, commonly with eight or more Segments when complete. A.1.2 appendices: Distinctly differentiated pro- longations of the body of an arthropod; e.g. legs, wings, antennae, urogomphi. A.1.3 bristles: Fine but stiff hairs of any length present
14、 on the cuticle of insects. NOTE 3 Special sensory hairs are called setae. A.1.4 caterpillars: Larvae of Lepidoptera spp. NOTE 4 Butterfly or moth chrysalis is the pupal Stage. is the aduit Stage and the Aal.5 cephalic capsule: Sclerous part of an exuvia which once contained the head of a larva. Als
15、o called head capsule. A.l.6 ecdysis; exuviation: Casting of the juvenile insect cuticle, thereby allowing growth to take place. NOTE 5 The old cuticle is called the exuvia. A.l.7 exuviae: Cuticle cast during ecdysis. A.1.8 false legs; pro-legs: Fleshy extensions of the lower part of the abdomen of
16、some Iarvae, some- times with a crown of fine hooks (crochets) of Chitin. These help with attachment to the Substrate, and in movement. Lepidopterous larvae have at least two pairs of false legs, towards the rear of the body. A.1.9 feelers: A non-scientific term for the sensory appendages situated o
17、n the head capsule of insects. They may be close to the eyes, and called antennae, or associated with the mouthparts and generally called palps. A.l.10 insects: A class of animals within the Phylum Arthropoda, some of which are recognized pests of stored foodstuffs. A.l.ll juvenile stages: The pre-a
18、dult stages of in- sects; e.g. egg, larva, nymph and pupa. NOTE 6 This term is most often applied to the active stages of larva and nymph. A.1.12 labrum: The upper lip, covering the mouth opening, of some larval and adult insects. A.1.13 mandibles: Toughened (sclerotized) mouth- Parts of insects, us
19、ed for dilaceration or grinding of food. A.l.14 mites: Very small arthropods belonging to the class Arachnida, sub-class Acarina, often living in large numbers. A.1.15 pericarp: Extemal envelope of seeds which forms the bran after the grain has been crushed and the flour separated. A.1.16 scales: Br
20、istles which have evolved into flat structures resembling fish scales and which cover Parts of the body of certain insects, in particular the wings of Lepidoptera spp. A.1.17 Stage: The state of development of an insect or mite; e.g. egg, larva, nymph, pupa, adult. A.1.18 urogomphi: Pointed extensio
21、ns of the cuticle of the final adbominal Segment of some insect larvae. They are common, and sometimes diagnos- tic, features of many Coleoptera spp. NOTE 7 The abdominal extensions of a cockroach are called cerci. A.1.19 wing case; elytron: Hardened front wing of Coleoptera spp., used as fixed wing
22、 in flight and as protective cover for the membranous hind wing. A.2 Characteristics The distinction between vegetable and animal frag- ments is based on their general appearance and structural characteristics. The complete juvenile stages of insects and mites are easily recognized. Fragments of ins
23、ects and some heavily sclerotized mites range in colour from light brown to grey/brown and have a surface of shiny appearance, or of a Pattern of small knobs, Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/997254500
24、1 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 11050:1993(E) depressions, pits or regular striations. The food storage mites are usually translucent white. Frag- ments of vegetable origin have a matt appearance and are generally a
25、light reddish brown in colour. Those insect or mite fragments which are found will originate more often than not from the appendages (legs and antennae), or from particularly robust Parts of the body such as the mandibles. In the case of fragments from other Parts of the body (e.g. head, wing cases,
26、 abdomen, etc.), these tan be distin- guished by their translucent appearance and, in the case of the largest, by their construction which is in the form of juxtaposed plates. Unlike the fragments of the pericarp of seeds, which exhibit cellular Walls (visible at the magnifications indicated in the
27、pro- cedure) with thick cellulose membranes, no cellular structure tan be distinguished in the cuticle of in- sects upon examination under a microscope. The surface of insect fragments generally exhibits fine hollow spotting in an irregular Pattern, strewn with small circular depressions at the cent
28、re of which it is occasionally possible to distinguish the base of hairs or bristles (see figureA.l). Rodent hairs have an internal structure in the form of transverse black streaks of irregular shape oc- curring over the the entire length of the hair. These streaks may be more or less distinct, dep
29、ending on the state of digestion of the hair. However, human hairs and the fur of domestic animals exhibit a con- tinuous structure without streaks. Whole insect cuticles have the form of very thin fragments which may be quite large in size. They are quite easy to identify since they still carry hai
30、rs (bristles) or exhibit the characteristic forms of the Organ which they enclosed (cephalic capsule of the head, form of the limbs, circular hooks on the false legs of Caterpillars, etc.). 7 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NAS
31、A Technical Standards 1/9972545001 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 11050:1993(E) 100 pm 1 a) Rodent hairs 100 pm I Fragments of cuticle Mandible c) Coleoptefa 100 pm Labrum Scales d) Lepidoptera b) Fragment of seed epi
32、derm (bran) Urogomphi (larva) Antenna bpex) e) Mite Figure AA - Different types of fragment found on the filters Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/21/2007 08:47:10 MDTNo re
33、production or networking permitted without license from IHS -,-,- ISO 11050:1993(E) Annex B (informative) Example of a test report - Determination of animal impurities in accordance with ISO 11050 Identification of the Sample - Type of product: - Sample reference: - Date of reception of the Sample:
34、- Information concerning the method of sampling: - Date of analysis: Results of analysis Test Portion No. 2 3 t 4 1 5 t 6 (if rodent hairs or fragments of hairs are found) Rodent hair or fragments of hair Whole insects (larva, nymph or adult) Insect fragments (including butterfly scales), insect egg
35、s, whole mites and their fragments 0 bservations - Notes on the animal impurities n number of large insect fragments of more than 200 pm in size, = number of mites or fragments of mites. - Notes w on the operating procedure, n on the progress of the analysis. - Presence of impurities other than anim
36、al impurities (number per type of impurity). - Other observations. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without lic
37、ense from IHS -,-,- ISO 11050:1993(E) Annex C (informative) Diagram of the procedure 1 i 2 Suspension Acid hydrolysis , 4 Paraffin oil Test Portion - + Water +b / FB *: :. ; er? ;: . . . : . . f : * . - . .* Min the 3 HCI / 5 Water/aIcohoI (l/l) 5 Water/alcohol (l/l) Separation (slow) Gentle rinses
38、under vacuum Microscopic examination and quantification 10 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license fro
39、m IHS -,-,- ISO 11050:1993(E) Annex D (informative) Chronology of operations and timetable Stage 1: preparation of the apparatus and the test portion, hydrolysis with hydrochloric acid for 1 h 30 min to 2 h (see table below). Step Opetations Duration min. max. A Rinsing and drip-drying of glassware,
40、 covering of Containers with film, filtration of reagents. IO min 20 min B Homogenization of Sample, constitution of test portion, weighing. 5 min 10 min C Addition of water to test Portion, rinsing of glass rod and sides of beaker. 5 min 10 min D Stirring, addition of HCI, formation of starch Paste
41、, addition of Paraffin 1 h 10 1 h 20 I I Oil, boiling for 30 min, cooling. I I I Stage II: concentration of the impurities and rinsing of the aqueous Phase for 2 h 30 min (at least) to 3 h (av- erage for two test portions). (In particular, two Separations for 1 h, followed by one Separation for 30 m
42、in.) Stage Ill: rinsing of funnels, plus filtration and conditioning of the filters, taking from 30 min to 1 h. Stage IV: drying of the filters for 1 h in an oven, or for 10 min on the filter-holder using hot air. Stage V: interpretation of the filters - 1 h per filter (depending on the number of im
43、purities), or 2 h on average for two test portions. Summary Stage ShortestIlongest duration Total I II Ill IV V duiation Shortest duration 1 h 30 2 h 30 30 min 10 min Ih 5 h 40 Longest duration 2h 3h Ih Ih 2h 9 h 1) 1) The determination may be carried out over 2 days either by performing the hydroly
44、sis on the first day and the Separation overnight, or by performing the hydrolysis and Separation on the first day and the ex- amination of the filters on the second day. 11 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standa
45、rds 1/9972545001 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- This page intentionally leff blank Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/997254
46、5001 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- This page intentionally left blank Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for
47、 Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 11050:1993(E) UDC I664.641 .12 + 664.7621544.869 Descriptors: agricultura I products, impurities, microscopic analysis. plant products, food products, cereal products, wheat fiour, semolina, tests, determination, Price based on 11 pages Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/21/2007 08:47:10 MDTNo reproduction or networking permitted without license from IHS -,-,-
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