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1、BRITISH STANDARD BS 6898:1987 Specification for Glass centrifuge tubes for general laboratory use UDC 542.2.7:541.18.043.3:542.1 BS 6898:1987 This British Standard, having been prepared under the direction of the Laboratory Apparatus Standards Committee, was published under the authority of the Boar
2、d of BSI and comes into effect on 30 October 1987 BSI 11-1999 The following BSI references relate to the work on this standard: Committee reference LBC/25 Draft for comment 86/52987 DC ISBN 0 580 16185 4 Committees responsible for this British Standard The preparation of this British Standard was en
3、trusted by the Laboratory Apparatus Standards Committee (LBC/-) to Technical Committee LBC/25, upon which the following bodies were represented: Association of Scientific, Technical and Managerial Staffs British Laboratory Ware Association CLEAPSE Chemical Industries Association Department of Health
4、 and Social Security Glass Manufacturers Federation Institute of Medical Laboratory Sciences Institute of Science Technology Royal Society of Chemistry Amendments issued since publication Amd. No.Date of issueComments BS 6898:1987 BSI 11-1999i Contents Page Committees responsibleInside front cover F
5、orewordii 1Scope1 2Definition1 3Designation1 4Sizes1 5Material1 6Dimensions1 7Details of construction2 8Inscriptions2 9Package labelling2 Figure 1 Dimensions of tubes3 Table 1 Dimensions for tubes1 Table 2 Dimensions for the cone of conical bottom tubes1 Publications referred toInside back cover BS
6、6898:1987 ii BSI 11-1999 Foreword This British Standard has been prepared under the direction of the Laboratory Apparatus Standards Committee and deals with a product not previously covered by a British Standard. The standard has been prepared to provide a limited range of glass centrifuge tubes, wi
7、th either rounded or conical bottoms, for general laboratory use. The requirements in this standard are designed to provide tubes that: a) for swing-out head centrifuges, are strong enough to withstand the forces of an acceleration of up to 35 000 m/s2, i.e. 3 500 times the acceleration due to the e
8、arths gravity; b) for fixed angle head rotor centrifuges, will withstand up to 30 000 m/s2, in regular use. NOTECertain tougher glasses are capable of withstanding the forces of an acceleration in excess of these values. The tubes have been designed with sufficient ullage space to permit their use i
9、n centrifuges with swing-out heads. Centrifuge manufacturers recommendations should be followed when the tubes identified in this standard are used in fixed angle headed centrifuges. Requirements for laboratory centrifuges are specified in BS 4402. WARNING. Particular attention should be paid to the
10、 use of correct size of shield/bucket and cushion profile. Failure to observe these details will considerably reduce safe working limits. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct applicatio
11、n. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages 1 to 4, an inside back cover and a back cover. This standard has been updated (see copyright date) and
12、 may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. BS 6898:1987 BSI 11-19991 1 Scope This British Standard specifies requirements for a limited range of two types of glass centrifuge tubes for general laboratory use. The two types specifie
13、d are: a) graduated or ungraduated glass centrifuge tubes with a rounded bottom; b) graduated or ungraduated glass centrifuge tubes with a conical bottom. NOTE 1The two types are shown in Figure 1. The nominal volumes covered are 6 mL, 9 mL, 13 mL, 25 mL and 40 mL (see clause 3). NOTE 2The nominal v
14、olumes indicated are those representative of the actual volume below the fill line. Traditionally these were referred to as 5 mL, 10 mL, 15 mL, 25 mL and 50 mL. The glass centrifuge tubes specified in this British Standard are suitable for use with solutions of apparent density up to 1.2 g/mL at 20
15、C and are designed to withstand the forces due to an acceleration of 35 000 m/s2. NOTE 3The titles of the publications referred to in this standard are listed on the inside back cover. 2 Definition For the purposes of this British Standard the following definition applies. nominal volume volume belo
16、w the upper line see 8.1 c) above which the tube should not be filled 3 Designation The tubes shall be designated by their nominal volume in millilitres. 4 Sizes The requirements of this British Standard shall apply to the series of glass centrifuge tubes given in Table 1. 5 Material The glass centr
17、ifuge tubes shall be made from colourless medium wall thickness borosilicate glass 3.3 complying with BS 2598-1, or from soda-lime-silica glass or other glass of at least equivalent strength. NOTEThe tubes should be as free as possible from visible defects and reasonably free from internal stress. 6
18、 Dimensions 6.1 Actual volume The actual volume shall be 10 % of the nominal volume. The nominal volume shall be indicated as specified in 8.1 b) and as shown in Figure 1. 6.2 Length The length, h1, shall be as given in Table 1. 6.3 External diameter The external diameter and the tolerance on the ex
19、ternal diameter shall be as given in Table 1 and in BS 5895. Table 1 Dimensions for tubes 6.4 Wall thickness The wall thickness and the tolerance on the wall thickness shall be as given in BS 5895 for the corresponding medium wall tubing. 6.5 Height of cone In the case of the conical bottom glass ce
20、ntrifuge tubes, the nominal height, h3, of the cone from the base shall be as given in Table 2. Table 2 Dimensions for the cone of conical bottom tubes 6.6 Apex of the cone In the case of the conical bottom glass centrifuge tubes, the apex of the cone shall be not less than 0.2 times the internal ra
21、dius, r, of the tube, as shown in Figure 1. 6.7 Bottom of the rounded bottom tubes In the case of the rounded bottom glass centrifuge tubes, the internal radius, r, of the bottom of the tube shall be 0.5 times the internal diameter of the cylindrical portion, as shown in Figure 1. Nominal volume Len
22、gth (h1 in Figure 1) External diameter mLmmmm 6 9 13 25 40 75 2 110 2 110 2 110 2 110 2 14 14 17 22 28 Nominal volume Nominal height of cone from base (h3 in Figure 1) mLmm 6 9 13 25 40 18 18 18 23 23 BS 6898:1987 2 BSI 11-1999 7 Details of construction 7.1 General Except for the part of the tube de
23、signated h3 in Figure 1, the tube shall be cylindrical, and the ovality shall not exceed the limit given in BS 5895. 7.2 Top The top of each tube shall be smoothly finished at right angles to the axis, with a fire-polished end, or tapered to accommodate stoppers or screwed closures. NOTE 1Modificati
24、on to rim for closure purposes may significantly reduce the actual capacity of the tube. Other dimensions should not be changed. NOTE 2WARNING. Attention is drawn to BS 4402:1982, in particular clauses 13, 14 and B.2, when the centrifuging of potentially biologically hazardous or potentially chemica
25、lly hazardous materials is proposed. 7.3 Bottom 7.3.1 Rounded bottom tubes. The bottom of each tube shall be hemispherical. Its wall thickness shall be between 1 and 2 times the vertical wall thickness. 7.3.2 Conical bottom tubes. The bottom of each tube shall be conical. Its wall thickness shall be
26、 between 1 and 2 times the vertical wall thickness. 8 Inscriptions 8.1 General inscriptions The following inscriptions shall be marked on each tube. a) Material identification. Each tube shall bear an indication of the type of glass used in its construction. b) Volume identification. Each tube shall
27、 be marked to indicate its nominal volume. c) Upper line. To indicate the level above which it is essential not to fill the tube for use in a swing-out head centrifuge, each tube shall be permanently marked with a line 5 mm from the top of the tube, h2, as shown in Figure 1, except in the case of a
28、tube graduated as in 8.2 which has the upper graduation line between 5 mm and 10 mm from the top of the tube. 8.2 Additional inscriptions Graduated tubes, in addition, shall be permanently marked with a scale indicating the volume contained in the tube at each scale mark. The tolerance on this scale
29、 shall be 10 %. No graduation line shall be marked closer than 5 mm to the top of the tube. 9 Package labelling Each package of centrifuge tubes shall be marked with the following information: a) the number and date of this British Standard, i.e. BS 6898:19871); b) the manufacturers and/or vendors n
30、ame or mark; c) nominal volume; d) type, i.e. graduated or ungraduated, rounded or conical bottom; e) batch number. 1) Marking BS 6898:1987 on or in relation to a product represents a manufacturers declaration of conformity, i.e. a claim by or on behalf of the manufacturer that the product meets the
31、 requirements of the standard. The accuracy of the claim is therefore solely the responsibility of the person making the claim. Such a declaration is not to be confused with third party certification of conformity, which may also be desirable. BS 6898:1987 BSI 11-19993 Figure 1 Dimensions of tubes 4
32、 blank BS 6898:1987 BSI 11-1999 Publications referred to BS 2598, Glass plant, pipeline and fittings. BS 2598-1, Specification for properties of borosilicate glass 3.3. BS 4402, Specification for safety requirements for laboratory centrifuges. BS 5895, Specification for borosilicate glass tubing for
33、 laboratory apparatus. BS 6898:1987 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal
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