ISO-10718-2002.pdf
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1、INTERNATIONAL STANDARD ISO 10718 Second edition 2002-08-01 Reference number ISO 10718:2002(E) ISO 2002 Cork stoppers Enumeration of colony-forming units of yeasts, moulds and bacteria capable of growth in an alcoholic medium Bouchons en lige Dnombrement des units formant colonie de levures, de moisi
2、ssures et de bactries capables de se dvelopper dans un milieu alcoolique Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 06:54:46 MDTNo reproduction or networking permitted without license from I
3、HS -,-,- ISO 10718:2002(E) ii ISO 2002 All rights reserved PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the
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6、 the Central Secretariat at the address given below. ISO 2002 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from eit
7、her ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.ch Web www.iso.ch Printed in Switzerland Copyright International Organization for Standardization
8、Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 06:54:46 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 10718:2002(E) ISO 2002 All rights reserved iii Foreword ISO (the International Organization for Standardization) is a
9、 worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represente
10、d on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are draf
11、ted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
12、 Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO 10718 was prepared by Technical Committee ISO/TC 87, Cork.
13、This second edition cancels and replaces the first edition (ISO 10718:1993), which has been technically revised. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 06:54:46 MDTNo reproduction or net
14、working permitted without license from IHS -,-,- Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 06:54:46 MDTNo reproduction or networking permitted without license from IHS -,-,- INTERNATIONAL S
15、TANDARDISO 10718:2002(E) ISO 2002 All rights reserved 1 Cork stoppers Enumeration of colony-forming units of yeasts, moulds and bacteria capable of growth in an alcoholic medium 1Scope This International Standard specifies a method to enumerate the colony-forming units of yeasts, moulds and bacteria
16、 which can exist on cork stoppers and can grow in an alcoholic solution under certain conditions. This International Standard applies to cork stoppers which were submitted to the usual sanitizing procedures. 2Normative reference The following normative document contains provisions which, through ref
17、erence in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, this publication do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the
18、 most recent edition of the normative document indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standards. ISO 7218, Microbiology of food and animal feeding stuffs Gen
19、eral rules for microbiological examinations 3Principle Direct counting of colonies of living micro-organisms (yeasts, moulds and bacteria) by incubation in a cultural medium after extraction in an alcoholic solution charged with tartaric acid and followed by a membrane filtration procedure. 4Reagent
20、s and cultural media 4.1Physiological solution ( NaCl)1) or Ringer solution ()1) with the following composition: Sodium chloride Potassium chloride Calcium chloride 6H2O Sodium bicarbonate Final pH (obtained from the mixture) 1)This product is commercially available. 0,85 %1/4 X 2,25 g/l 0,105 g/l 1
21、2 g/l 0,05 g/l 7,00,2 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/23/2007 06:54:46 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 10718:2002(E) 2 ISO 2002 All rights re
22、served 4.2WLD (for counting bacteria) with the following composition. 4.3M-Green (for counting yeasts and moulds) with the following composition. 4.4Tartaric acid. 4.5Ethanol, . 4.6Surface-active agent. 4.7Triptone gel. 4.8Diphenyl. Reagents and cultural media should be stored according to the manuf
23、acturers instructions 5Apparatus Usual microbiological laboratory apparatus and, in particular, the following. 5.1Membrane filtration system. One of the membrane filtration systems described at 5.1.1 or 5.1.2 may be used. 5.1.1Sterile filtration system, ready to use, including a polypropylene funnel
24、 with a capacity of at least , a sterile membrane (porosity ), a sterile dish and a vacuum pump with a three-way cock to turn off the vacuum. NOTEThis system is commercially available. 5.1.2Traditional filtration system including a funnel with a minimum capacity of (of stainless steel, glass or poly
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