ISO-21149-2006.pdf
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1、 Reference number ISO 21149:2006(E) ISO 2006 INTERNATIONAL STANDARD ISO 21149 First edition 2006-03-01 Cosmetics Microbiology Enumeration and detection of aerobic mesophilic bacteria Cosmtiques Microbiologie Dnombrement et dtection des bactries arobies msophiles Copyright International Organization
2、for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/16/2007 09:06:13 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 21149:2006(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Ado
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5、e has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. ISO 2006 All rights reserved. Unless otherwise specified, no part of this publication may
6、 be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel.
7、+ 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2006 All rights reserved Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/16/2007 09:06:13 M
8、DTNo reproduction or networking permitted without license from IHS -,-,- ISO 21149:2006(E) ISO 2006 All rights reserved iii Contents Page Foreword iv 1 Scope . 1 2 Normative references. 1 3 Terms and definitions. 1 4 Principle. 2 4.1 General. 2 4.2 Plate count. 2 4.3 Membrane filtration. 2 4.4 Detec
9、tion of bacteria by enrichment. 3 5 Diluents, neutralizers and culture media 3 5.1 General. 3 5.2 Neutralizing diluents and diluents 3 5.3 Diluent for the bacterial suspension (tryptone sodium chloride solution). 4 5.4 Culture media 4 6 Apparatus and glassware 6 7 Strains of microorganisms 7 8 Handl
10、ing of cosmetic products and laboratory samples . 7 9 Procedure 7 9.1 General recommendation 7 9.2 Preparation of the initial suspension 7 9.3 Counting methods 8 9.4 Enrichment 9 10 Counting of colonies (plate counts and membrane filtration methods). 9 11 Detection of growth (enrichment method). 9 1
11、2 Expression of results . 10 12.1 Method of calculation for plate count. 10 12.2 Interpretation. 10 12.3 Examples . 11 12.4 Detection after enrichment 13 13 Neutralization of the antimicrobial properties of the product 13 13.1 General. 13 13.2 Preparation of inoculum 14 13.3 Validation of counting m
12、ethods 14 13.4 Validation of the detection method by enrichment. 15 13.5 Interpretation of validation results 15 14 Test report . 16 Annex A (informative) Other neutralizing diluents . 17 Annex B (informative) Other diluents. 19 Annex C (informative) Other culture media. 20 Annex D (informative) Neu
13、tralizers of antimicrobial activity of preservatives and rinsing liquids. 23 Bibliography. 24 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=Aramco HQ/9980755100 Not for Resale, 04/16/2007 09:06:13 MDTNo reproduction or networking permitted w
14、ithout license from IHS -,-,- ISO 21149:2006(E) iv ISO 2006 All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
15、ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates
16、 closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards.
17、 Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this
18、document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 21149 was prepared by Technical Committee ISO/TC 217, Cosmetics. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee
19、=Aramco HQ/9980755100 Not for Resale, 04/16/2007 09:06:13 MDTNo reproduction or networking permitted without license from IHS -,-,- INTERNATIONAL STANDARD ISO 21149:2006(E) ISO 2006 All rights reserved 1 Cosmetics Microbiology Enumeration and detection of aerobic mesophilic bacteria 1 Scope This Int
20、ernational Standard gives general guidelines for enumeration and detection of mesophilic aerobic bacteria present in cosmetics, by counting the colonies on agar medium after aerobic incubation, or by checking the absence of bacterial growth after enrichment. Because of the large variety of cosmetic
21、products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method
22、 has been otherwise validated. If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiol
23、ogical risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. 2 Normative references The fo
24、llowing referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 21148:2005, Cosmetics Microbiology General instruct
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