ISO-20963-2005.pdf
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1、 Reference number ISO 20963:2005(E) ISO 2005 INTERNATIONAL STANDARD ISO 20963 First edition 2005-04-01 Soil quality Effects of pollutants on insect larvae (Oxythyrea funesta) Determination of acute toxicity Qualit du sol Effets des polluants vis-vis des larves dinsectes (Oxythyrea funesta) Dterminat
2、ion de la toxicit aigu ISO 20963:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performin
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5、tariat at the address given below. ISO 2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the add
6、ress below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2005 All rights reserved -,-,- ISO 20963:2005(E) ISO 2005 All ri
7、ghts reserved iii Contents Page Forewordiv Introduction v 1 Scope1 2 Normative references .1 3 Terms and definitions.1 4 Principle.2 5 Test environment3 6 Reagents3 7 Apparatus.4 8 Procedure.4 8.1 Test design 4 8.2 Preparation of test mixture 5 8.3 Preparation of control container .6 8.4 Food additi
8、on 6 8.5 Introduction of the biological material6 8.6 Test conditions and measurements6 8.7 Reference substance6 9 Expression of results7 9.1 Calculation.7 9.2 Expression of results8 10 Validity of the test .8 11 Test report8 Annex A (informative) Example of breeding technique for Oxythyrea funesta
9、.9 Annex B (informative) Results of the collaborative trial carried out in France11 Bibliography .12 ISO 20963:2005(E) iv ISO 2005 All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The wor
10、k of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governme
11、ntal, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The m
12、ain task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. A
13、ttention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 20963 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological
14、methods. -,-,- ISO 20963:2005(E) ISO 2005 All rights reserved v Introduction This International Standard describes a method for the determination of the acute toxicity of contaminated soils and chemicals to the larvae of Oxythyrea funesta, a phytophagous coleopteran (Scarabaeidae, Cetoniinae) with w
15、ide geographic distribution (Europe, North Africa and the Middle East). Oxythyrea funesta has many characteristics which make it suitable for soil quality monitoring or testing effects of chemicals: ecological relevance: this type of organism contributes in many ways to soil structure by stimulating
16、 soil aeration and drainage; the first stages of development, i.e. incubation of eggs, larval cycle and pupation, are underground; the larvae of Oxythyrea funesta are tolerant to modifications of the test substrate granulometry; this species can be bred under controlled conditions. -,-,- INTERNATION
17、AL STANDARD ISO 20963:2005(E) ISO 2005 All rights reserved 1 Soil quality Effects of pollutants on insect larvae (Oxythyrea funesta) Determination of acute toxicity 1 Scope This International Standard describes a method for the determination of the effects of contaminated soils and substances on the
18、 survival of the larvae of Oxythyrea funesta. The larvae are exposed to the pollutants by cuticular and alimentary uptake. For contaminated soils, the effects on the survival are determined in the test soil and in a control soil. Depending on the objectives of the study, the control and dilution sub
19、strates (dilution series of contaminated soil) are either uncontaminated soil comparable to the soil sample to be tested or artificial soil substrate. Effects of substances are assessed using a defined artificial soil substrate. This International Standard is not applicable to volatile substances, i
20、.e. substances for which Henrys constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,001 33 Pa at 25 C. NOTE This method does not take into account the possible degradation of the substances or pollutants during the test. 2 Normative reference
21、s The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 10381-6, Soil quality Sampling Part 6: Guid
22、ance on the collection, handling and storage of soil for the assessment of aerobic microbial processes in the laboratory ISO 10390, Soil quality Determination of pH ISO 11268-1, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 1: Determination of acute toxicity using artificial
23、 soil substrate ISO 11269-2:1), Soil quality Determination of the effects of pollutants on soil flora Part 2: Effects of chemicals on the emergence and growth of higher plants 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 1) To be published. (R
24、evision of ISO 11269-2:1995) -,-,- ISO 20963:2005(E) 2 ISO 2005 All rights reserved 3.1 lethal concentration 50 LC 50 median lethal concentration of test substance or percent dilution of contaminated soil, which kills 50 % of the test organism within the test period 3.2 lethal concentration x LCx co
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