《ISO-14715-1999.pdf》由会员分享,可在线阅读,更多相关《ISO-14715-1999.pdf(8页珍藏版)》请在三一文库上搜索。
1、A Reference number ISO 14715:1999(E) INTERNATIONAL STANDARD ISO 14715 First edition 1999-10-01 Oil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L. Huile essentielle de thym thymol, type Espagne Thymus zygis (Loefl.) L. Copyright International Organization for Standardization Provid
2、ed by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/25/2007 06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 14715:1999(E) ISO 1999 All rights reserved. Unless otherwise specified, no part of this publication may be
3、 reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization Case postale 56 CH-1211 Genve 20 Switzerland Internetisoiso.ch Printed in Switzerland
4、ii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which
5、a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matt
6、ers of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standar
7、d requires approval by at least 75 % of the member bodies casting a vote. International Standard ISO 14715 was prepared by Technical Committee ISO/TC 54, Essential oils. Annexes A and B of this International Standard are for information only. Copyright International Organization for Standardization
8、Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/25/2007 06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,- INTERNATIONAL STANDARD ISOISO 14715:1999(E) 1 Oil of thyme containing thymol, Spanish type Thymus zygis
9、(Loefl.) L. 1 Scope This International Standard specifies certain charac- teristics of the oil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L., in order to facilitate assessment of its quality. 2 Normative references The following normative documents contain provisions which, throu
10、gh reference in this text, constitute provisions of this International Standard. For dated references, subsequent amendments to, or revisions of, any of these publications do not apply. However, parties to agreements based on this International Standard are encouraged to investigate the possibility
11、of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain registers of currently valid International Standards. ISO/TR 210, Essential oils General rules fo
12、r packaging, conditioning and storage. ISO/TR 211, Essential oils General rules for labelling and marking of containers. ISO 212, Essential oils Sampling. ISO 279, Essential oils Determination of relative density at 20 C (Reference method). ISO 280, Essential oils Determination of refractive index.
13、ISO 592, Essential oils Determination of optical rotation. ISO 875, Essential oils Evaluation of miscibility in ethanol. ISO 1272, Essential oils Determination of phenols content. ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles
14、 for presentation in standards. ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils. 3 Term and definition For the purposes of this International Standard, the following term and definition apply. 3.1 o
15、il of thyme containing thymol, Spanish type essential oil obtained by steam distillation of the flowering tops of Thymus zygis (Loefl.) L., of the Lamiaceae family, growing mainly in Spain 4 Requirements 4.1 Appearance Clear, mobile liquid. 4.2 Colour Traditionally from reddish brown to very intense
16、 brown (almost black). NOTE The essential oil obtained by distillation in a stainless-steel kettle has a much lighter colour. The distillation of red thyme leads to the so-called white thyme with similar characteristics. 4.3 Odour Characteristic, aromatic, phenolic (thymol), with a slightly spicy ba
17、se. 4.4 Relative density at 20 C, d20 20 Minimum:0,910 Maximum:0,937 4.5 Refractive index at 20 C Minimum:1,494 0 Maximum:1,504 0 Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/25/2007
18、06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 14715:1999(E) ISO 2 4.6 Optical rotation at 20 C Between 1 and 6. NOTE Generally laevorotatory; frequently impossible to measure due to its colour. 4.7 Miscibility in ethanol, 80 % volume fraction, at 20 C It shal
19、l not be necessary to use more than 3 volumes of 80 % ethanol to obtain a clear solution with 1 volume of essential oil. 4.8 Total phenols content Minimum volume fraction: 38 % Maximum volume fraction: 56 % 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromat
20、ography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. Table 1 Chr
21、omatographic profile ComponentMinimum % Maximum % -Thujene0,21,5 -Pinene0,52,5 Myrcene12,8 -Terpinene0,92,6 -Terpinene411 p-Cymene1428 trans-Sabinene hydratetr0,5 Linalool36,5 Terpinen-4-ol0,12,5 Methyl ether of carvacrol0,11,5 Thymol3755 Carvacrol0,55,5 -Caryophyllene0,52 NOTE The chromatographic p
22、rofile is normative, contrary to typical chromatograms given for information in annex A. 4.10 Flashpoint Information on the flashpoint is given in annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard
23、 to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, d20 20 See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 80 % volume fraction, at 20 C See ISO 875. 6.5 Phenols content See ISO 1272. Stir for 6 h
24、 using a magnetic stirrer. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/99725
25、45001 Not for Resale, 04/25/2007 06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,- ISOISO 14715:1999(E) 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the oil of thyme containing thymol, Spanish type Thymus zygis (Loefl.) L. Pe
26、ak identification 1-Thujene 2-Pinene 3Myrcene 4-Terpinene 5p-Cymene 6-Terpinene 7trans-Sabinene hydrate 8Linalool 9Terpinen-4-ol 10Methyl ether of carvacrol 11Thymol 12Carvacrol 13-Caryophyllene Operating conditions Column: fused silica capillary; length 50 m; internal diameter 0,20 mm Stationary ph
27、ase: polydimethylsiloxane Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 220 C at 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Linear velocity of carrier gas: 0,35 m/s appro
28、x. Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/25/2007 06:49:14 MDTNo reproduction or networking permitte
29、d without license from IHS -,-,- ISO 14715:1999(E) ISO 4 Peak identification 1-Thujene 2-Pinene 3Myrcene 4-Terpinene 5-Terpinene 6p-Cymene 7trans-Sabinene hydrate 8Linalool 9Terpinen-4-ol 10Methyl ether of carvacrol 11Thymol 12Carvacrol 13-Caryophyllene Operating conditions Column: fused silica capi
30、llary; length 25 m; internal diameter 0,20 mm Stationary phase: polyethyleneglycol 20 000 Film thickness: 0,25 m Oven temperature: temperature programming from 65 C to 200 C at 5 C/min Injector temperature: 240 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volum
31、e injected: 0,2 l Linear velocity of carrier gas: 0,35 m/s approx. Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale,
32、 04/25/2007 06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,- ISOISO 14715:1999(E) 5 Annex B (informative) Flashpoint B.1 General information For reasons of safety, transport companies, insurance companies, people in charge of safety services, etc., require informati
33、on on the flashpoint of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was hard to find a single method for standardization purposes, given that: essential oils are varied and their chemical co
34、mpositions differ to a large extent; the volume of the sample needed for certain test equipment is incompatible with the high price of essential oils; there are different types of equipment that satisfy the desired objective, but users cannot be obliged to use one type of equipment rather than anoth
35、er. Consequently, it was decided to give a mean value for the flashpoint in an informative annex in each International Standard, for information purposes, in order to meet the requirements of the interested parties. If possible, the method by which this value was obtained should be specified. For fu
36、rther information see ISO/TR 110181). B.2 Flashpoint of essential oil of thyme containing thymol, Spanish type The mean value is +60 C. NOTE Obtained with “Setaflash“ equipment. _ 1) ISO/TR 11018, Essential oils General guidance on the determination of flashpoint. Copyright International Organizatio
37、n for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/25/2007 06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,- ISO 14715:1999(E) ISO ICS 71.100.60 Price based on 5 pages Copyright International Organization for Standardization Provided by IHS under license with ISO Licensee=NASA Technical Standards 1/9972545001 Not for Resale, 04/25/2007 06:49:14 MDTNo reproduction or networking permitted without license from IHS -,-,-
链接地址:https://www.31doc.com/p-3777310.html