ISO-3946-1982.pdf
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1、International Standard 3946 INTERNATIONAL ORGANIZATION FOR STANDARDIZATIONWEIKJJYHAPOAHAR OPTAHM3AWlR fl0 CTAHAPTM3AWlMRGANISATION INTERNATIONALE DE NORMALISATION Starches and derived products - Determination of total phosphorus content - Spectrophotometric method Amidons, fkcules et produirs dtke e
2、a8ibration CeWQ and H-E deler- mination wittair-l 2 i-i. -j-ake 383 aliquot porab-? (se?e E? -tabkji and introduce it im0 a w nd conical f-lask. Atid vdaler 8Q easch mf he %QVQD flasks so thaak ehe mtaB volun3e is approximately 30 in%. T;X” Plaee khe seven dlasks in dx boihng water baah 5.69 fsr 10
3、min. Usinq the spectropp.BOforQQler, delermine the absorbance a-t 825 nm Qf -this soiL.!timi. Cool 10 arnbiefif PemperaQure by imsnersing thf3 fkks in the cooling bash (5.5). Transfer the csntents of ahe %Iasks quaw- tifatively 8 tB-9 50 nd one-rnark mhmetaic flasks E.1) = Dilute %gz the marks wiah
4、wafer and r!-liX Read f?-On7 number 0-f Id-Ie caBibraaior1 micrsgrams cif cubwQ ph0sph Isee 6.13 the csrrespondin QWS. 9 Using the spectrophobxaaeter I599, delmM-7 Phe absorbance 5 nm of each sf the six ssutions, using he 10khsr-i frsm he flask withsul the standard sdution as Phe reference. Plot the
5、 cahbration eurve giving he m.mber sf n-krograms of phospkarus as a funcfi0n sf the abssrbanee. Carry out placing U-i a bIank Best e Test psrtion in paralld by water. demv-ninatisn, PQ- Carry out two deterrnirxhsns on the sa-ne Test sample (6.2). blix =the sarnple PhorsughDy. -,-,- ISO 39464982 (El
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- ISO 3946 1982
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