JIS-K-0350-80-10-2005-ENG.pdf
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1、J IS JAPANESE INDUSTRIAL STANDARD Translated and Published by Japanese Standards Association JIS K 0350-80-10 :zoo5 (JIWA/ JSA) Testing method for detection of iron bacteria in industrial water ICs 13.060.25; 13.060.70 Reference number : JIS K 0350-80-10 : 2005 (E) PROTECTED BY COPYRIGHT 8s Copyrigh
2、t Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 08:25:27 MDTNo reproduction or networking permitted without license from IHS -,-,- K 0350-80-10 : 2005 Foreword This translation has been made based
3、on the original Japanese Industrial Standard established by the Minister of Economy, Trade and Industry through deliberations at the Japanese Industrial Standards Committee according to the proposal of establishing a Japanese Industrial Standard from Japan Industrial Water Association (JIWA)/ Japane
4、se Standards Association (JSA), with a draft of Industrial Standard based on the provision of Article 12 Clause 1 of the Industrial Standardization Law. Attention is drawn to the possibility that some parts of this Standard may conflict with a patent right, application for a patent after opening to
5、the public, utility model right or application for registration of utility model after opening to the public which have technical properties. The relevant Minister and the Japanese Industrial Standards Committee are not responsible for identifying the patent right, application for a patent after ope
6、ning to the public, utility model right or application for registration of utility model after opening to the public which have the said technical properties. Date of Establishment: 2005-01-20 Date of Public Notice in Official Gazette: 2005-01-20 Investigated by: Japanese Industrial Standards Commit
7、tee Standards Board Technical Committee on Environment and Recycling Policy JIC K 0350-80-10 : 2005, First English edition published in 2005-05 Translated and published by: Japanese Standards Association 4-1-24, Akacaka, Minato-ku, Tokyo, 107-8440 JAPAN In the event of any doubts arising as to the c
8、ontents, the original JIS is to be the final authority. O JSA 2005 All rights reserved. No part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. Printed in
9、Japan “/AT PROTECTED BY COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 08:25:27 MDTNo reproduction or networking permitted without license from IHS -,-,- K 0350-80-10 2005 1 2 3
10、4 5 5.1 5.2 6 6.1 6.2 6.3 7 Contents Page Scope Normative references Definitions Common matters . Sample . Sampling . Handling of sample . Testing method Instrument and apparatus . Pretreatment of sample Operation Expression of test results Attached Table 1 Normative references . Annex 1 (informativ
11、e) Method of confirmation for the cell in iron bacteria (dyeing method) Annex 2 (informative) Example of mention of appearance frequency in every kind of iron bacteria . 1 1 1 1 1 1 3 3 3 3 4 5 6 7 9 ti PROTECTED BY COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license wit
12、h JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 08:25:27 MDTNo reproduction or networking permitted without license from IHS -,-,- JAPANESE INDUSTRIAL STANDARD JIS K 0350-80-10 2005 Testing method for detection of iron bacteria in industrial water 1 Scope This Ja
13、panese Industrial Standard specifies the testing method for judge- ment of existence of iron bacteria in industrial water qualitatively by using a micro- scope. 2 Normative references The standards listed in attached table 1 contain provi- sions which, through reference in this Standard, constitute
14、provisions of this Stan- dard. The most recent editions of the standards (including amendments) shall be applied. 3 Definitions 0101, JIS K 0102, JIS K 0211, JIS K 0550 and the following definition apply. For the purposes of this Standard, the definitions given in JIS K 3 . 1 the generic term of bac
15、teria which inhabit widely in ground water and undercurrent water containing much iron component and have such prop- erties that iron (II) or its compound is oxidized to iron (III), and accumulated in the inside of bacteria form and precipitated on the outside of bacteria form Iron bacteria, which i
16、s brown cell and the precipitation form is dif- ferent in every genus, has the characteristic to form a particular external form. These external form can be directly judged by con- firming by using microscope. For the representative, the following two geneses are given. Gallionella ferruginea shows
17、generally a twisted ribbon shaped external form of 1 pm to 1.5 ym in width and the cell of iron bacte- ria exists at its tip. For Leptothrix ochracea, long bacilli stand in a line in long linear sheaths of approximately 2 pm in width. In ad- dition to this, several kinds of iron bacteria with charac
18、teristic ex- ternal form have been reported. The propagation of iron bacteria causes the various obstacles such as the production of brownish rusty water or slime, the blockage of filtration layer in water purifying process. iron bacteria Remarks : 4 Common matters The common matters shall be as fol
19、lows: 4 . 1 Glassware The glassware, which are specified in JIS R 3503 and JIS R 3505, shall be generally used. When special instruments are required, an example of it is illustrated or explained respectively. 5 Sample 5 . 1 Sampling 5 . 1 . 1 Reagents The reagents shall be as follows: PROTECTED BY
20、COPYRIGHT Copyright Japanese Standards Association Provided by IHS under license with JSALicensee=IHS Employees/1111111001, User=Wing, Bernie Not for Resale, 03/13/2007 08:25:27 MDTNo reproduction or networking permitted without license from IHS -,-,- 2 K 0350-80-10 : 2005 a) Formaldehyde solution F
21、ormaldehyde solution 37 % (mass percentage) (1) ( 2 ) specified in JIS K 8872 shall be used. Notes (1) The handling of formaldehyde solution shall follow the related laws and regulations and care shall be sufficiently taken. (2) 1,5-Pentane dial (glutaraldehyde) solution 125 % (mass percentage) may
22、be used instead of formaldehyde solution. 5.1.2 Instruments The instruments shall be as follows: a) Sample container bottle, 100 ml to 1 O00 ml. Glass bottle with grounding stopper or clean polyethylene b) Water sampler Heyroth type water sampler. An example is shown in fig- ure 1. I t I I I I I I l
23、 8 I I I I I l I l I I I I I ,.A- , / / A : B : C : D : E : F: G : H : I : Glass bottle (100 ml to 1 O00 mi) Stopper of bottle Chain Chain for stopper opening Chain for fastening the holder of bottle Holder of bottle Portable container Lid of portable container Sinker Figure 1 An example of Heyroth
24、type water sampler and portable container 5.1.3 Operation The sampling shall be as follows: Sampling of surface water The surface water from lakes and marshes, riv- ers, waterway, water storage tank, etc., when direct sampling is possible, shall be sampled by using a sample container. When direct sa
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