HBV变异对乙肝病毒标志物和DNA定量的影响.doc
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1、HBV变异对乙肝病毒标志物和DNA定量的影响 来源:本站 |作者:奚华新, 蒋跃明 |时间:2006-11-28 9:50:13 | 浏览: 387 字号选择大 中 小 摘 要 目的: 了解无锡地区HBV前CC基因变异情况,探讨基因变异对乙肝病毒标志物(HBVM)及HBV DNA定量的影响。方法: HBVM用时间分辨荧光定量试剂检测;HBV DNA定量PCR以德国Roche Lightcycler 荧光定量扩增仪检测;HBV DNA前CC测序使用 德国Roche Mag NA Pure LC 全自动核酸提取,加样系统; 美国Beckman Coulter CEQ 8000 Genetic An
2、alysis System。结果: 92例乙肝患者血清中有41例(446)发生前CC区nt1896位变异,41例中有34例HbeAg阴性,7例HbeAg仍为阳性;以nt1896位是否变异分组,变异组和未变异组HBV DNA定量无显著性差异。结论: nt1896位变异导致HBeAg转阴,HBV病毒变异对HBVM有决定性的影响,但该变异对HBV复制无影响。 关键词 DNA序列分析; 基因变异; HBVM DNA定量 Affection of Gene Mutation to Serological Markers and Quantity of DNA of Hepatitis B Virus X
3、I Huaxin, JIANG Yueming (1.The Blood Center of Wuxi,Wuxi Jiangsu 214021; 2.Wuxi Hospital for Infectious Disease,Wuxi Jiangsu 214007, China) Abstract Objective: To report HBV preCCgene mutation in wuxi area,to determine the affection of gene mutation to serological markers and quantity of DNA hepatit
4、is B virus.Methods: Serological markers of hepatitis B virus were detected by timeresolved fluorescence immunoassay. Quantitative analysis of HBV DNA PCR was performed by Roche Lightcycler fluoresencent quantitative amplification analyzer which made in Germany. HBV DNA preCCgene fragments were direc
5、tly sequenced by Roche MagNA Pure LC automatic nucleic acid getting, sampling system made in Germany and Beckman CoulteCEQ8000Genetic Analysis System made in the U.S. Results: Fortyone from nintytwo(4192)patients had preCCgene mutation at site at nt1896.there thirtyfour HBeAg were negative and seven
6、 HBeAg were positive.Gene mutation at site at nt1896 didnt affect virus replication. Conclusion: How HBeAg getting negative was dependant on nt1896 site gene mutation.Gene mutation at site at nt1896 didnt affect hepatitis B virus replication. Key words DNAgene fragments analysis;Gene mutation;Serolo
7、gical markers of hepatitis B virus;Quantitative analysis of HBV DNA HBV感染在临床可产生不同的肝脏疾病谱,过去多以HBV免疫学标志物来判断病毒感染状况1。但随着分子生物学技术的发展,研究发现肝脏疾病谱不仅与宿主的免疫有关,也与病毒本身密切相关,即HBV 本身也存在变异。对HBV变异的判断,最可靠和精确的方法是从病人体内检出HBV DNA,用DNA测序法来确定病毒的变异,目前对于HBV的变异的研究比较集中在关于前C区及S基因区的变异。本文结合92例乙肝患者的PCR定量、免疫学标志物定量结果以及DNA前CC区测序结果报道如下。
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- 关 键 词:
- HBV 变异 乙肝病毒 标志 DNA 定量 影响
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