BS-7011-3.1-1989 ISO-8255-1-1986.pdf

BRITISH STANDARD BS 7011-3.1: 1989 ISO 8255-1: 1986 Consumable accessories for light microscopes Part 3: Cover glasses Section 3.1 Specification for dimensions and optical properties ISO title: Optics and optical instruments Microscopes Cover glasses Part 1: Dimensional tolerances, thickness and optical properties NOTEIt is essential that this Section is read in conjunction with the general information given in BS 7011-0 “General introduction”, which is published separately. UDC 681.723:681.7.024:681.785.6:(083.74) Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7011-3.1:1989 This British Standard, having been prepared under the direction of the Laboratory Apparatus Standards Committee, was published under the authority of the Board of BSI and comes into effect on 28 February 1989 © BSI 10-1999 The following BSI references relate to the work on this standard: Committee reference LBC/33 Draft for comment 84/65139 DC The committees responsible for this British Standard are given in Part 0 ISBN 0 580 16823 9 Amendments issued since publication Amd. No.Date of issueComments Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7011-3.1:1989 © BSI 10-1999i Contents Page National forewordii 0Introduction1 1Scope and field of application1 2Reference1 3Requirements1 4Marking1 Publications referred toInside back cover Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7011-3.1:1989 ii © BSI 10-1999 National foreword This Section of BS 7011 has been prepared under the direction of the Laboratory Apparatus Standards Committee. It is identical with ISO 8255-1:1986 “Optics and optical instruments Microscopes Cover glasses Part 1: Dimensional tolerances, thickness and optical properties”, published by the International Organization for Standardization (ISO). ISO 8255-1 was prepared by Subcommittee 5, Microscopes, of Technical Committee ISO/TC 172, Optics and optical instruments, with the active participation of the UK. This Section supersedes some of the requirements given in BS 3836-1:1974, which are deleted by amendment. The other Parts and Sections of this standard are listed in Part 0. Terminology and conventions. The text of the International Standard has been approved as suitable for publication as a British Standard without deviation. Some terminology and certain conventions are not identical with those used in British Standards; attention is drawn especially to the following. The comma has been used as a decimal marker. In British Standards it is current practice to use a full point on the baseline as the decimal marker. Wherever the words “part of ISO 8255” appear, referring to this standard, they should be read as “Section of BS 7011”. Additional information. Referring to clause 4 e), for microscope cover glasses which comply also with Section 3.21) of this British Standard it is sufficient for each package of cover glasses to be marked with the number of Part 3 of this British Standard, i.e. BS 7011-32). A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages 1 and 2, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Cross-reference International StandardCorresponding British Standard ISO 7944:1984BS 7017:1988 Specification for reference wavelengths for optics and optical instruments (Identical) 1) In preparation. 2) Marking BS 7011-3 on or in relation to a product represents a manufacturers declaration of conformity, i.e. a claim by or on behalf of the manufacturer that the product meets the requirements of Section 3.1 and 3.2 of this standard. The accuracy of the claim is therefore solely the responsibility of the person making the claim. Such a declaration is not to be confused with third party certification of conformity, which may also be desirable. Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7011-3.1:1989 © BSI 10-19991 0 Introduction This part of ISO 8255 lays down dimensions and specifies optical quality requirements in order to guarantee the quality of observation. The data given in this part of ISO 8255 are applicable to most products in use and have been adapted to take into account the relevant national standards in force. This part of ISO 8255 contains requirements for dimensional tolerances, thickness and optical properties, whereas quality requirements and test methods related to the material will be dealt with in a future part of ISO 8255. 1 Scope and field of application This part of ISO 8255 specifies requirements for dimensional tolerances, thickness and optical properties for microscope cover glasses used for transmitted light microscopy in the visible spectral range3). NOTEThis part of ISO 8255 does not cover requirements for suitability for fluorescence microscopy. 2 Reference ISO 7944, Optics and optical instruments Reference wavelengths. 3 Requirements 3.1 Dimensional tolerance The tolerance on nominal length, width or diameter of cover glasses shall be ± 0,5 mm. 3.2 Thickness Cover glasses shall conform to the following thickness ranges: NOTE 1Microscope manufacturers, for purposes of optical design, use 0,17 mm as the combined thickness of cover glass and mounting medium, measured from the top surface of the cover glass to the top surface of the specimen being observed. NOTE 2In addition to the above No. 1 and No. 1-H cover glasses, other thicknesses are available, such as(0,17 mm) and No. 2 (0,17 mm) which may be used for some purposes. Highest optical quality, particularly with large aperture objectives, may not be obtained with these thicknesses.4) 3.3 Optical properties The optical properties of cover glasses shall be as follows: NOTEThe principal refractive index, ne, is the refractive index of light at the green mercury e-line (Æe= 546,07 nm; see ISO 7944). This wavelength is close to the maximum sensitivity of the eye and has been used as the principal wavelength of optical computation for some time. The Abbe number, Ée, is calculated using the following formula: where 4 Marking The packaging of microscope cover glasses conforming to this part of ISO 8255 shall be marked with the following information: a) the thickness, No. 1 or No. 1-H; b) the dimensions; c) the average number of cover glasses per package or weight; d) the manufacturers or suppliers name or mark, and the country of origin; e) a statement that the cover glasses comply with the requirements laid down in this part of ISO 8255. 3) The visible spectral range is defined as being the range from 400 to 760 nm. No. 1 (general purpose): 0,17 mm No. 1-H (high performance): 0,17 mm 4) Note 2 is only of a cautionary nature, but does not form part of the standard. 0 0,04 0 0,02 11 2 +0,02 0,01 +0,08 0 Principal refractive index: ne= 1,525 5 ± 0,001 5 Abbe number: ve= 56 ± 2 neis the principal refractive index; nF½is the refractive index of light at the blue cadmium F½-line (ÆF½ = 479,99 nm); nC½is the refractive index of light at the red cadmium C½-line (ÆC½ = 643,85 nm). Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI 2 blank Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7011-3.1:1989 © BSI 10-1999 Publications referred to See national foreword. Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 7011-3.1: 1989 ISO 8255-1: 1986 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions. It is the constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using this British Standard would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front cover. Tel: 020 8996 9000. Fax: 020 8996 7400. BSI offers members an individual updating service called PLUS which ensures that subscribers automatically receive the latest editions of standards. Buying standards Orders for all BSI, international and foreign standards publications should be addressed to Customer Services. Tel: 020 8996 9001. Fax: 020 8996 7001. In response to orders for international standards, it is BSI policy to supply the BSI implementation of those that have been published as British Standards, unless otherwise requested. Information on standards BSI provides a wide range of information on national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services are also available which give details on all its products and services. Contact the Information Centre. Tel: 020 8996 7111. Fax: 020 8996 7048. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details of these and other benefits contact Membership Administration. Tel: 020 8996 7002. Fax: 020 8996 7001. Copyright Copyright subsists in all BSI publications. BSI also holds the copyright, in the UK, of the publications of the international standardization bodies. Except as permitted under the Copyright, Designs and Patents Act 1988 no extract may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, photocopying, recording or otherwise without prior written permission from BSI. This does not preclude the free use, in the course of implementing the standard, of necessary details such as symbols, and size, type or grade designations. If these details are to be used for any other purpose than implementation then the prior written permission of BSI must be obtained. If permission is granted, the terms may include royalty payments or a licensing agreement. Details and advice can be obtained from the Copyright Manager. Tel: 020 8996 7070. Licensed Copy: London South Bank University, London South Bank University, Fri Dec 08 12:55:15 GMT+00:00 2006, Uncontrolled Copy, (c) BSI