BS-5711-18-1979.pdf

BRITISH STANDARD BS 5711-18: 1979 Methods of Sampling and test for glycerol Part 18: Detection of sugars NOTEIt is recommended that this Part of BS 5711 be read in conjunction with the general information given in BS 5711-0 “General introduction”, issued separately. UDC 661.188.1:547.426.1:543.064:547.455/.458 Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 This British Standard, having been prepared under the direction of the Chemicals Standards Committee, was published under the authority of the Executive Board and comes into effect on 30 November 1979 © BSI 12-1999 The following BSI references relate to the work on this standard: Committee reference CIC/6 Draft for comment 75/53579 DC ISBN 0 580 10999 2 A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages 1 and 2, an inside back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on the inside front cover. Amendments issued since publication Amd. No.Date of issueComments Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 © BSI 12-1999i Contents Page 1Scope and field of application1 2References1 3Principle1 4Reagent1 5Sampling1 6Procedure1 7Test report1 Publications referred toInside back cover Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI ii blank Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 © BSI 12-19991 1 Scope and field of application This British Standard describes a colorimetric method for detecting the presence of sugars in glycerol. 2 References The titles of the publications referred to in this standard are listed on the inside back cover. 3 Principle Heating a test portion with a dilute sulphuric acid solution of urea and tin (II) chloride for 15 min on a steam bath. Development of a marked colour (usually brown) indicates the presence of sugar. 4 Reagent The reagents used shall be of a recognized analytical grade. Distilled water complying with the requirements of BS 3978 or water of at least equal purity shall be used. Reagent solution. Dissolve 4 g of urea and 0.2 g of tin (II) chloride by heating with 10 ml of approximately 400 g/l aqueous sulphuric acid. NOTEIt is usually convenient to make at least 30 ml of solution. 5 Sampling Prepare the laboratory sample as described in Part 2 of this British Standard. 6 Procedure Introduce 4 drops of the sample into each of two test tubes, 150 mm by 12.5 mm. To one tube add 1 ml of the reagent solution and to the other add 1 ml of distilled water, as a control (note 2). Immerse both tubes in a steam bath and examine after 15 min (note 3). If a marked colour develops in the tube containing the reagent, sugars are present. NOTE 1A marked colour shows the presence of 0.25 % or more of sugars. For methods of determination of sugars, see clause 7 of Part 22 of this standard. NOTE 2If preferred, a reference mixture can be made of several crude glycerols known to be sugar-free. The control is prepared by using 4 drops of the reference mixture in the second tube and following the procedure described in clause 6. NOTE 3The time chosen is sufficient to give a significant colour change even with low concentration of sugars, though in many cases 5 min would suffice to develop a colour. 7 Test report 7.1 Report the result as either “sugars detected” or “no sugars detected”. 7.2 The test report shall include the following particulars: a) a reference to this British Standard; b) the result and the method of expression used; c) any unusual features noticed during the test; d) any operations not included in this British Standard or regarded as optional. Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI 2 blank Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18:1979 © BSI 12-1999 Publications referred to BS 3978, Water for laboratory use. BS 5711, Methods of sampling and test for glycerol. BS 5711-2, Methods of sampling. BS 5711-22, Additional methods of test (non-specification). Licensed Copy: sheffieldun sheffieldun, na, Mon Dec 04 15:12:28 GMT+00:00 2006, Uncontrolled Copy, (c) BSI BS 5711-18: 1979 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by amendment or revision. 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