1、Identificationandmo1.ecu1.archaracterizationofPowderyMi1.dewofC1.ematismanshuricaCausedbyErysipheau.HaiJi;NXu.YimXHuiOv.NingHw.JunEanF.DearTC(1.ofPImKP(h1.f.CdkjeofPUniProcti(XkShcn)nAgrhzUkUrn1.Un1.VCTXhy.Sbenyw5.1.ijiming.H0$i66.ChinaC1.eniatumt/Hihitncai,upr.isaperennia1.herbintheKanuiKruIacvaeta
2、n1.y.whichhasbeenusedasuVu1.uab1.c(raditiow1.Chinesemodici,Between2012and2013.aPOWdCrymi1.dewcausingsignifkan1.damagetnp1.antsofGmanshuricaintheMa1.icimi1.HerbGardeninShenyangAgricu1.tura1.Universi1.y.Uaoningprovince.China.haedredascircu1.artovu1.ar.thu.WhMec。IOnieon1.xthsidesof1.ea%*eax1.youngstems
3、especia1.1.yontheadaxia1.surface.Andeventua1.1.yinfectionsusua1.1.ycausedye1.1.owingofthe1.eave%am!Tnii1.fnrrndtinnundprematurektUnu1.ctissueswaskn1.ifedasEmipheucui1.eae(Er5spaks.AscomyaHab%edonInorPhOiQgiCa1.CharaCtCriJiCsPhy!gcncicana1.ysisbasedonrDNASshowedthu(theSNi2strainfa1.1.inIbeNamewe11-M
4、pottedchfe.whichisc1.。W1.yrehedIoErysiffJie蚓I1.Negiae.pathogenici1.ytoorigina1.hostwasCOnAfTnodthroughinocu1.ationc.pcrincncs.Tor1.ofpnvderymikkwcmC.ManShMriCQOIga1.byE.tqui1.eiatinChina.Introduction/Ittt1.iItSNSPJngDDoC.1.rwrWcmIhercrthcas(ofChina.Thedriedrootandrhizonsofthep1.antsnamed“WeiIingxiaE
5、intheCInDCSCPhanMCoPOCEispopu1.ar1.yusedaxanUntiinf1.amnuitory,ant(unr.ana1.gesicagentinUaditiuiu1.Cbinewnedicinefor1.horiadsofyeainAsi4StiiSPeIJ20OdInA1.IRUMof2012.NPOwdCTymi1.dewnC.QIanShUriaIwmfirMobservedinMedicina1.IIcrbGardenofShenyangAgriCUhUni1.Universityin1.iaoningprovince.Chinxduringinvest
6、igationofp1.antdivca%conC.ntanhuricwdeymi1.dewbsenedonC1.etnatiinMHi1.11trainChiiwandseiupabaseforSCiCnUnCcontro1.ofthedisease.Theribosoma1.DNAinterna)CninscribedspacerI!TSregionhasbeenusefu1.fordiscriminatingfngi(thespeciesfcvd(i81.1.ftifcnui!ykgenetana1.ysisofuderyImkkvI1.KI4Hr2001.k05tJamesH.CUnn
7、1.nton2003.200.Wrag.HiXX).k1.esofC1.emuiisrruuuhitricantecedw1.hxwderymi1.dewwerecbtauedfrntheMcdiciiw1.HcrtiGardenofShenyangAgriehUni1.University.1.iaoningprovic.China.Themorpho1.ogyofthep;I1.bQgCn、wereexaminedfrcUmii1.eriahuing1.ightmicroscopyaspreviousdescnbed心1e1.a2001).!)NAextractionandamp1.1.f
8、1.cathnOrri)NAITSfttt1.2t1.Ghk.KTA.Sn.GS.Cumi1.mJ.H.TuImuIkuS.,Huc.I.B0b,1.NcPCSn,Y.11JRmgmA.Y.f2X1.1.INdgvsoftwomM*fmp.CAif1.Mtr*atvrzmpnew；fidO枚KTWMinfntjnjcindrfcrcMpct(Ikr*rv1.MgUM1.III*6M-6Q1*ttN3上Wmxkkr“ku出tfd*w厢MwW,r*MIbffCxmmIIXXgof，R，E1.1.TwrthiuUeoMi1.ikw即MoUiCiXohmc01.epaw山Ms9州KErpeaoutj8
9、scnsuioJ.Funna1.Oversf/.2004.16:110.Meh:M1.rMcnti1.kutiMEe1.,uda*i*nf%xT.1.uruc.B.$J,YtTAa11bu.S.1.2(KOEgfMhvfrwymkSffspecKesdoroU311mDcuUrKiCra3300AWN(JeVa1.uggiNorrveMOfPpurc.M1.VPOWeymMIUngi*tt11$上NxtooidCSOqg3mwsityCtrDNAsre3IracQtwdspacerCX1.rac1.edIrwnCOnddar1.cieetchecacfSeVOraIr-Ktt!IO:fiu*v
10、CBMw*cmIntrAkptk4h1.1.1.1.IafeffrMnmm1.i11cdFrUXmer1.1.1.x111DNAMimp1.cswerePrCPdrCdhyco1.1.ectingCkiQmheci;IITc1.Nuy;iF1.irataax1.SuuimuTakamaisu/HDdyoungodcrynk1.wmye1.iaUSingSwri1.Cbuhcj.Jankovics.2001.Thenuc1.earrDNAregion%panmngtheITSk5.8SndS2rRNgenewasUmPIHia1.byPO1.ymeraWchainreaction(PCR).Pr
11、imersITS!FTS4We1.Eusedfortheam1.ifkaion.whichwasperformedinatow!reactionvo1.umeof501.inc1.udingthefo1.1.owingreagents:5仙IM)XPCRbu11cr,2.01.DNAIcmp1.a1.ct41.MgCk(IOniM),1.()1.Qra1.dIprimer(ITSIandITS4),0.61.T1.KfDNApo1.ymerase5.0U1.).1.01.dNTP(dATP.dCTRdGTRdTTP.K)nMeach),35.41dd1.1.：O.1.1.abovervagen
12、txwerejxirvhaxa!fmmSangnnBiotechno1.ogy1.id(Shanghai.China).ThePCRCyChngconditionsWCrC94c*Cfor3minfb1.1.outxiby34cycksof94vCIwImin455cCforImin,72C1.r1.5min,andahna1.extensionUcpof72,CforXIn1.ninaS100DrMIhennaIcyc1.er(Bio-Rad1.akraiones.1.fr.USA).Sequencinganddataana1.ysisDNAwuenccofthreercp1.kate%we
13、reobtainedusingtheTianGcnDNAsecurcP1.antkitSequencingoftheITSregionswasdonebySangonBiokgyInfaiI1.IhUn(NCB1.Bcihesdii.MDivreobtainedTaMci).ITSsequencesSCrCa1.ignedwithprevious1.ypub1.hhedsequences1.11nc1.ose1.yreb1.cdPnWdcrymi1.dewpccicmaintain1.inGenBank.Phy1.ogeneticana1.yseswerePCffQmIedusingMEGEv
14、ersion4.1WiIhIbCcighbor-joiningmethods.RCMI1.KSymptomF.1.SymptoemofPOWCkry11ik1.cwOaC1.cmatumwMMa:SymPMxTMin6dd;b:y11tdciuonkuf)Thefngiinfectedmain1.y(be1.eaves,thesu1.ksundpetio1.escana1.sobeinfectedinSCVCginfections.Syinpionntni1.ia1.1.yappearedascircu1.artoirregu1.ar,1.ht.whi1.eco1.onies,whichsub
15、sequent1.ydeve1.opediniodense,whitehyphaeauiconkiiaont1.uppersurfacesoftheyoungIe:IVgandynungMemespecia1.1.yontheahxia1.vurhce.andPoMdCrycn1.onicvmergedocoverentire1.eaves.A1.I1.iesanenine,twwco1.oniesstaduccesive1.ytoheother1.eaves.Intheeaseofsevereinfections,cok)nicwerea1.soobservedontheundersurfa
16、ceof1.ea,esak1.bo1.eivdonyongMef11.A1.nfeeied1.eavesbecameSray1.esionsIxriwai1.ifunga1.c4onics.Asthediseaseprogressed,infectionsusua1.1.ycausedye1.1.owingofthe1.eavesJirx1.PC1.in1.candprematuredctb1.iation.Onoccasion、severe1.yma1.formationonyoung1.eavesatxipetio1.e%ven?observed.FhviHv.nunerouC1.eivt
17、oi1.iecbi3eatvdmidieIxxhsutfacesof1.eaves.Ma1.ksandpc1.io1.cs.MrpTCRiCa1.ChanICtariHimtjyitre5旧改RUQnCkwatbnwmrwra(a：C1.ciUo1.hcciiiENXMedmin”CIiUn1.vkaf;b:CIcifoNchi.aciundappendage:c:CgidgPhOrCandcxnidu;d:ASCimdMXnpcg1.Onthe1.iving1.eavesu1.ksadpetio1.es,superficia1.hyphaethu*wa1.kd.hya1.ine,e(ate.
18、straightof1.exuous.3.5-8.8m(average5.6m)wide.ConidiophoresdecdfromtheexICnW1.PrinKIrymyce1.iumwerehya1.ine,erectmus.darkamtxrrandspherica1.withudiameterof96.1Ic190.6m(average137.1m)/Xpe11dije%were2.4to6.9mwide,usua1.1.yUiibiunched.benkd.wideninga(axttwiceofItiewaya1.ongtheICngthofthec1.ciMothccia.60
19、2co336,6m(average144.8m).EachchasmothccimINWIyCnn1.ainedeightsta1.kedaurusvwithavc11ge59.536.5m.achascusccnUi11e1.4-6ovoid-e1.1.ipsoidascosporesthatmeasured163io292m9.9to17.5m(average22.212.8m).tt1.iII】：川MW=中碘于*七*我为公主9.中耀H的，(HBpha*e)f1.ttt1.I2:Th，fr供Mrr“皿JrNrD.Vat.IununcuUcofana11MMpandte1.e11r)are
20、covsten(WiIhthedescriptionsoftheF1.orafungommSiniCOrUm1中国料学俄中国胞广植物“1.ift辑委员会.1987；Iu1.itoriu1.Kiiiin1.of1.ra%pnre-prxx1.ucingp1.antsofCinnC诲AcademyofSciences.I:IthCSginm,inuauciusOfPowdCrymi1.dewOnCmandmricaWaSdeterminedU)bdErysipheOqVi1.egiQeDCinChinaPhy1.ogenetic11My%hToconfirmtheidcnticaonofthecr
21、a1.fngu.theinterna1.transcribedSPiUXraTS)rvionofrDNwitsamp1.i1.ia1.IrinEF*mc11TSI.1TS4;HuIyNenCCddinx1.1.y.ThertMI1.1.inE619-bpsequencev*akxxtedinGenBank(AeveMionN.JX2*MX62).Thesequencewasmost(I(X%)simi1.artothatofErysip1.te(tqui1.eguciso1.ateAq2(AccessionNo.EUanSTO).ThePhy1.ogcnciictreeswereobtaine
22、dusingMEGE4.1withtheCighborJoiningmethods,andshowedtk1.IhcItCXfromCnunhuricainChinatbr111.auniceax1.we1.1.supportedc1.afet!utgroupedwith(1.ie.uui1.eiacreferenceiso1.ate.*11icm?ivsuInIix1.ieatedIhMIbeiso1.atesofE.(iquHegiaearcconspecific.Onthebasisof11xrphohgica1.characeris1.icN.ITSsequencesimihri1sa
23、miphy1.ogeneticana1.ysis.IbCiso1.ateW处idctit*icdasEaiMeWiIegMeDC.PU1.hORcnkH1.PatMgenetyWaScontained1.htug!iniuh1.iD11bygenii)dttugconidia(C1.ds1.o1.hec-MrAsa好PorCS)onto1.eavesoffivehea1.thy,pottedC.nanshuricap1.ants.Fivenon-inocu1.odp1.ants*crviasacontro1.Irciitment.P1.ant%weremaintainedinagreenhni
24、weat22*3Cto25Candabout70%to80re1.ativehumidityundernatura1.day!h(.DPiCa)symptomsofPowXkrymi1.dewOni11ocuktcdp1.antswerercrx1.ucc1.after7*I(Mays.Whc皿“contro1.p1.antsrenuuedhea1.thy.TbefungusPreSe1.NnIheiMxru1.aedp1.antsvasidenica1.nxw)in1.$ea1.1.ytothu(origina1.1.yobservedonthediscucdp1.ants,fu1.fi1.
25、1.ingKochspostu1.ates.DicmonC.nMniuriaImsbecomeWidCIycu1.tivatedinrccvnyearsasaChinesetraditiona1.be山tnnrtheii%t.C1.ir,IntheproductionHCId、/Pn1.hhtchM1.J1.jnfnnc1.c.2OI3)Rndhck中川)三ttR3kNMMIkIq、k.201(tarctheInaindiseaseOnC.如-力*rim.7OMknojcc.thisstudyisthefi11itfoma1.rcp1.ogica1.Charac1.crizf1.tionofi
26、so1.ates,andcombinedmobo1.ogi1.Chan1.CIeriSticscqenccofrDNAS.andpathogenici1.yIcMstodemonNIraICthatthecausa1.agentofpowderymi1.dew1.neasureandcu1.tura1.practicesfortheredctionofthedisease.ErysiphcMi1.cfiacisacn11wnPOWdcrymi1.dewKndhasbeen(Iis1.ributcc1.inJapan.Indi凡Fin1.and.Po1.and.Eng1.and.Gcnnanya
27、ndChina,andmain1.infectedthep1.antsofAqM1.e*，Aconituzn.Anetnnc.C1.tha.C1.enwfix.CopiistPu1.wii1.1.atRanuncwtMtT1.M1.icirumandsoon卜国科学浣中国抱广植1匆志编刊委员会，19X7；Iu1.t1.ona1.boardofHnnisporcrx!cingp1.antsofCiscAcademyofSciences.1987*I(JameSH.CunnEgIOnU003.20W(T.ZxZJankovicmJm)causing(Xiux1.ef)mi1.dewunAfMfty
28、nateacuden,Mi1.dewofC.nanhuricuwasquitekw.butwhichsharp1.yincreasedin2013.withaverageof78%.Withinfccionsourcesaccumu1.ation.EryXPhT(Mu1.cii(ichasjrcii1.pcMcn1.ia1.tobecomeanimpnrUn1.Pa1.hnXCnofC.manhurica.CsuequenUy.i(istecexsan,tofu11!wstudyn.fttt”i：，a的n中BIn1.f检构石翱t员会上.PIMAt*Hccafucip9)M1.I1.6:CUMK
29、必OnJH1.avweAC.PascccIGUM1.gCmft)owxneDNAK由ffarCft9d*8gq8ECV3rtcH1.hnEryRicacuicf1.iaeSe勿HajJ.Funga1.D.vorar,2004,16:110.Miikva&ain1.ihMtk4“utuikuu1.CT71.atianfrPa1.br4n(j.JCD).2.421-4289XEIVI*:Wifiu*trtnmAmp1.tf1.cdSCEnII*nth1.*iMidRdn1.hnwUh1.nfvc1.intuPIimIScci11IKh1.1.rdtfEr.*i;6e4qd1.cyi1cifitjt
30、dcIkRiMuaoi1.Md:.Aaft1.ruUvuaP1.*1.Rdhky.Onthebasisofthemrphn1.ojicyChiinic1.eristicxofthefungiOhyrVCdOathep1.ants”ndun0htiontests,u,edeierniwdthatthedH3沅wascausedbyE.agiti1.egiaShxrhIWnotprevious1.ybeenreportedcausa1.agentsofpowderymi1.dewsonC.manshtriainChina.RrfrrenctM2OJ5.29-292ItttI9:Fin1.CiuIa
31、AqfmIim(H14KISHISP.J1.ANGDAN.DONGCX.1.a1.UgnansfromtherootsandrhizomesdCtema1.ismansuncaJJZeHschrm0rNakJerscu110B,AK)Urna1.Orchemica1.sciences.2006.611O):1299-1303.springer教我好/t川文献：TreerpenddsaporinsCommuna1.ion15.Oema1.osideCAthtcrpenodQ1.电fromtherootsof：hma11cu11andmatis(Ciastsg11shu11caRUPRjITS1.
32、5TCTAGGTGAACCTGCGGV共ID个城MITSIF：5CGGTCATTTAGACGAAGTAA丁共22个ITS4：STCeTCCGCTTATTGATATGCT队20个靴丛ITS5.5XjAAGTAAAAGTCGTAACAAGG3,共22个气塞,进化，a用序外就Ihe行EU047570ErysbeaquHegexAqegiaAY4528O1E.aquHegtaGexOematiSooAY452804Eg向电浴eexNQexaAY452805E.aquiiegeexRanuncukis9DQ335569E.aMgtaeexCathafWuhUSJN003594E.aqui1.egiae
33、exCasuarina99AB000939E.friesnvar.daurtcaGU252368E.heradeiAY870864E.OufchracCCDQ359698Neoerysiptiega1.eopsisFig.2.Neighbor-joiningtreeshowingthephy1.ogeneticre1.ationshipamongEnsiphecti1.enkieis1.atesinieedfromtheIISrDNregion.BoOtSIraPva1.uesbasedon100Orep1.icationsareindicatedabovethebranches,andIhCsca1.ebarrcprvscnts0.02nuc1.eotidesubstitutionspersite.I,ryiphcqui1.rficvar.ranuncu1.iGrcv.)ZhenX&Chen.AbKgCt
