1、pH对铀胞生长和成分含直的影响Biomassandproductionofsecondarymetabo1.itesince1.1.suspensioncu1.turesofDictainiiiisdasycarpusasaffectedbythemediumpHObaCUwne（黄柏B1.）,raxine1.1.one（MW）,imnin（柠检苦素）Diet;Imnine（白鲜餐）目的：1 .评价PH对生长量、细胞死亡率、成分种类和含量的影响：2 .PH对/态氮和钱态翅的影响。3 .培养时间对PH的影响内M1.p1.antmateria1.andestab1.ishmentofce1.1.s
2、uspensionCeuSUSpenSiCn的培养；前期已建立的体系（引用发表文章2.GrowthcurvepH,2-10,利用磷酸般冲液或其他物质精确调整.测定指标：Phe1.wtype（形态、颜色、质地、Cc1.1.viabi1.ity）:Growth（FW%DW）：Contents（种类和含量：”N的含量（NH4+和NO3.的含量）；PH变化3Extractionand（antiicationofsecondarymetabo1.ites4.Statistica1.ana1.ysisDiscussion：Biomassandproductionofsecondarymetabo1.ite
3、since1.1.suspensioncu1.turesofDictamnusdasycarpusasaffectedbythemediumpHObacunone(黄柏VI),fraxine1.1.one(WM)IImonin(fNBiffft)Dktamnine(白鲜破)AbstractKeywords:Introduction写作思路：Materia1.sandmethodsP1.antmateria1.Estab1.ishmentofca1.1.usandce1.1.suspensioncu1.turesGrowthcurveTimeaftersubcu1.ture加入法导子的时间，0,
4、7.14,21dExtractionandquantificationofsecondarymetabo1.ites引用自己文章ExtractspreparationandquantitativeHP1.Cana1.ysesofactivecompoundswereperformed,accordingtotheproceduresdescribedprevious1.y引自己3t文章)Statistica1.ana1.ysisA1.1.experinena1.datawerestatistica1.1.yana1.yzedbyonewayana1.ysisofvariance(ANOVA)u
5、singtheprotected1.est-significant-difcrencc(1.SD)test(P0.05)fcandwerepresentedbyaveragestandarderror(SE).Resu1.tsanddiscussionGrowthkineticsofce1.1.suspensioncu1.tures一种PH的生长曲线（CK=PH5.8）,（不同pH,每个PH值1个表）生长量（FVV和DW折线图）、CCI1.Viabi1.i1.y（折线图）、pH（折线图）、NH4+含量和No3-含E（折线图）Cu1.tarrtime(d)FrCShWeightD巧WeightCd1.1.abi1.ity(%)PHNH44ftNO3-M01O.2O.O310.20.035IO15202510.20.0310.20.0330Ce1.1.viabi1.ity,加入每个处理的连续动态细胞照片.多少天后测定呢？是否考虑培养Md后满定.PH处理+成分种类和含量PContent%Ofactivecmpounds(mDW)ObacunoneFraxine1.1.one1.bnoninDktamnine处理I处理210.20.0310.20.03IOJ0.0310.20.03处理35.8处理5处理6处理7
