1、蝴电悬浮体系的建立及成分测定Estab1.ishmentofCe1.1.suspensioncu1.turesofDictainiuisdasycarpusfortheproductionofbioactiveComP(HIndS(Dictaminine)Obacunone(黄柏Jraxine1.1.one(样),1.im11wthcurve(kinetics)1 -8weeks5 .Deterninationofce1.1.biomassI1.irough0.45umstain1.essste1.1.sieveioac1.ivecompounds(Dictamiiiiiw)Estab1.is
2、hmentofCe1.1.suspensioncu1.turesofDictamnusdasycarpusandbiosynthesisofactivecompounds(Dictaminine)AbstractKeywords:Introduction写作思路：药用植物介绍(是个重妥的药用植物，作为传统中药；功效：活性)主要成分是植物本身含，低(Zusua1.1.yvery1.ow)f1.Recent1.y9重要成分Ahasattractedinterestedduetoitsstrong的功效，活性，治疗的疾病，A在植物上veryminorproductionThereforea1.ter
3、nativeexp1.orationof*isimportant,的前人研究进展hasbeenreportedin*本文研究内容Materia1.sandmethodsP1.antmateria1.andestab1.ishmentofce1.1.suspensionInf1.uenceofPGRsonbiomassandtheProdUCtionofbioactivecompoundsOptimizationofcu1.tureconditions(1) Mediastrength：1/2、1/3、1/4、I.OMS(2) Inocu1.umdensity：2.55.10.15.20.25g
4、1.FW(3) Carbonsource(concentration)1.23,4,5.6,7%(4) Photoperiod:Darkt16-1.ight,24-1.ight(5) Initia1.pH:4.0,5.0,556.0.6.5.7.0.8.0.9.0.10.0(6)Mg2+Concentrationt370mg1.(7)NH4Concentrations1.650mg1.Growthcurve(kinetics)Determinationofce1.1.biomassQuantitativeana1.ysisofbioactivecompounds引用自己文章Extractspr
5、eparationandquantitativeHP1.Cana1.ysesofactivecompoundswereerfo11ed,accordingtotheproceduresdescribedprevious1.y引自己ft*文章)Statistica1.(Data)ana1.ysisA1.1.experimenta1.datawerestatistica1.1.yana1.yzedbyonewayana1.ysisofvariance(ANOVA)usingtheprotectedIeaSt-SignifiCanI-difference(1.SD)test(P0.05).andwe
6、representedbyaveragestandarderror(SE).Kesu1.tsanddiscussion生长素Conccntni1.inContentsofacthecompounds!mgDw)CYIIStat1.INOtMiCUnihFraxindhmc1.innin1.kUmniH*24D0.00.110.20.05IO.2O.O31020.0310.20.0310.20.031020.03Pakye1.1.ow.Itugik0，1.O2.0DKydMw,cixnptc1.5.0NAA0.00.10、1.02.05.0IBA0.00.10，1.02.05.0分裂索（固定2.
7、4-D为2.0m1.）ConCentriItionImg/11Frc*ihWeightJVeht(ContentsofactiveCOinPOUndMm田DW)Ce1.1.statusOImcuimiwKmuwUnvIJmaninIMctamnineKIN0.00.11O.2O.O310.20.03IO.2O.O31020.0310.20.03ojo.Pa1.cyd!ow.fragi1.eOJ1.02.0Daik,t!1.uw.CDmpOC1.5.0BAP0.()0.10.51.02.05.0Mediastrength序号MSStrengthFrexhWeightDr，Weight(gCont
8、cntxofactivecContentsofacthtrEnpoUndSimagDWOi1.!UUMOhucunoncFnixinc1.1.one1.imninDictnmnineI2.5025.00IO.2O.O310.20.0310.2i0.0310.210.03IOJ0.03IO.2O.O3Pa1.c),c1.1.ow.fragi1.e310.0415.0520.0625.0Carbonconcentration号CarbonConcentmtin(%)FFhWeigh!(f)DryWeht(C.,(nten1.soactiecCe1.1.%ta1.uxObavunoneFraxine
9、1.1.oneIJmoninOidamnineI02I10.20.0310.20.0310.20.03IO.2O.O31.criFFhWCiKht1.f)DryWeightContentsofactiveCofnPOUndq1.n晖DWCe1.1.3UNOhjiciinoncFraxifw1.1.onc1.imoninDictumnincI0.0020.2030.3710.20.03IO.2O,O3IO.2O.O3IO2O.O310.20.03IO.2O.O3Pa1.cs1.1.oW.fragi1.e40.6050.8061.00NH4CIConcentration序号Mirenen1.rat
10、ioContcntxofactivecCe1.1.statusObacunoneFriixhwUonvIJ11MninDkr1.amnineICK(MS)20.80IO.2O.O31O.2O.O3IO.2O.O3IO.2O.O310.20.03O.2O,O3Pa1.cxr1.1.ow.fragi1.e320.642.0052.5063.00注，外加入KNO3（摩尔等量）保持N6离子浓度不交，用NHKI升NHJ浓度应外加入KNo31650+80.0434（修帔）”01.10（气化候）2.ftS41.,此时保证了硝平窝.MS中正常NH4NO3（NH4,）浓度t1650+80.0434（硝K）=20
11、6mM克与廉尔换尊1克数+原子量（分子）=物Je的量（m川效）比如.8=氨气的物期的量=X/16=OSnwIK、O3分子量101.1（”NHQ分子量53.491NH4NO3分子重80.0454NH4+ConcentrationJXNH4：IConoen1.ratinttJFreshWeight(K)DryWeightConknUofacti%cCompoundsimDW)Cd1.statusOhiiciinoncFraxifw1.1.oncIJ11wnin1.)k-t*mnincI0.0020.801020.0310.20.0310.20.031020.0310.20.0310.20.03Pn1.cXv1.1.ow.fragi1.e31.6542.0052.5063.00注：保持、Oa离子浓度不变，用NHVI提升、H/浓度Gro1hcurve(kinetics)序号Cu1.tureT1.meiwkiFreshWcht(g)DryWcfcht(M)Contentsofad1.vecompounds(mggDW)Ce1.1.staticObacunoncFraxindhMic1.imoninDictnmnincII223310.20.031O.2O.O310.20.03IO.2O.O31O.2O.O3IO.2O.O3Pakye1.1.ow,fragi1.e4455667788
