1、营养元素对生物量和成分的提高Inf1.uenceofnutrientmanipu1.ationsonbiomassgrowthandproductionofhioactivecompounds（Dictamininc）ince1.1.suspension（ca1.1.us）cu1.turefDic1.amiiiisdasycarpusObacunone（黄柏B1.）JraXine1.Ione（格VI）,1.imonin（柠普索）Dictamnine（白鲜碱）目的：1 .评价大量元素（NH4NO3、KNO3、CaCI2.MgSO4、KH2PO4）的影响：2 .评价NH4-N和N03-N比例对生长
2、量和含量的影响。内容：1. P1.antmateria1.andestab1.ishmentofce1.1.suspensionCa1.IUS的培养，前期已建立的体系（引川发表文条）ce1.1.suspensioncu1.ture（用原配方）t2.0gFW=350m1.250m1.=J过滤=反显SUbCUIIUrC2. EfTcctofvaryingconcentrationsofmacroe1.ementsinthecu1.turemediumMacroe1.ements,NH4NO3,KN03,CaC12.MgSO4,KH2PO4设置浓度：FOIdS（原元素含量的0.0、0.5、1.0、1
3、5、2.0倍）3. EffectsofnitrogensourceonbiomassgrowthandProdUCtionofbioactivecompounds使用药品：NH4CI和KNo3设置处理：0.0、0.5、1.0、1.5、2.0fo1.dsNH4CI组合1.OfoIdKNO31.0fo1.dNH4CIfi0.00.5、1.0、1.5、2.（1fo1.dsKNO34. Determinationofce1.1.1.）iomass5. DeterminationofNII*、和、O3NConcentration弁考i2009-Prgcnsourceonbiomassgrowthand
4、productionobioactivccompoundsDeterminationofce1.1.biomassDeterminationofNH4N和NO3NConcentrationQuantitativeana1.ysisofbioactivecompounds引用自己文章ExtractspreparationandquantitativeHP1.Cana1.ysesofactivecompoundswereperformed,accordingtotheproceduresdescribedprevious1.y引自己ft*文章)Statistica1.(Data)ana1.ysis
5、A1.1.experimenta1.datawereSiaiisiica1.1.yana1.yzedbyonewayana1.ysisofvariare(ANOVA)usingtheprotectedICaSt-SignifiCanI-difference(1.SD)test(P().05).andwerepresentedbyaveragestandarderror(SE).Resu1.tsanddiscussion大量元素dtwContentsofactbecompoundsmg/gDW)Ce1.1.staticObaCUnwFraxindkMKaIJmonin1.kUvnniMNH4NO
6、10.0IO.2O.O3IO.2().O3K2O,O310.20.0310.20.03IO.2O.O3Pa1.cyd1.ow.fragi1.e0.51.01.52.0Darkyc1.1.nw.cxnpc1.KNo)0.0IO.2O.O310.20.03IOJ0.0310.2i0.0310.210.03IQ0.03Pa1.eye1.1.ow.Itugi1.e0.51.01.52.0CaCk0.0IO.2O.O310.20.03IO2O.1O.2O.O31020.03IOJO.(BPa1.cye1.1.ow,fragi1.e0.51.01.52.0MgSO40.00.20.030.20.03IO.
7、23IO.2O.O3JO.2O,O3IO.2O.O3Pa1.cye1.1.ow,fragi1.e0.51.01.52.0KmPo40.00.20.03I0.20.0310.20.03IO.2O.O3IO.2O,O3IO.2O.O3Pa1.cye1.1.ow,f11gi1.c0.51.01.52.0氮源比例NHU(Xt1.BBB)NOy(tj三8Frv*hWDnWeight1.gCfontenUoctieccnpund1.11DW)Cc1.sta1.esObacuMH?Fraxine1.hmeUmOnInDictaninhw0.01.00.20.03IO.2O.O310.20.031O.2O.O
8、31O.2O,O3IO.2O.O3Pa1.cye1.1.ow,fragi1.e0.51.01.01.01.51.02.01.0Darkye1.1.ow,compact0.0u.o1.00.010.20.0310.20.051320.0310.20.0310.20.03Pakye1.1.ow.Iiugi1.e1.00.51.01.01.01.51.02.0DeterminationofXH4-N和N()3-NConcentrationNHr(XUaM1.NOy(ti三(k1.SdKk1.ISd2(X125dKk1.0.51.01.01.0i.51.02.01.00.0O(O1.OOX1.1.00.51.01.01.0.51.02.0
