ISO-21571-2005.pdf
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1、 Reference number ISO 21571:2005(E) ISO 2005 INTERNATIONAL STANDARD ISO 21571 First edition 2005-02-15 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction Produits alimentaires Mthodes danalyse pour la dtection des organisme
2、s gntiquement modifis et des produits drivs Extraction des acides nucliques ISO 21571:2005(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded ar
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5、ating to it is found, please inform the Central Secretariat at the address given below. ISO 2005 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, wit
6、hout permission in writing from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2005 All ri
7、ghts reserved ISO 21571:2005(E) ISO 2005 All rights reserved iii Contents Page Forewordiv Introduction v 1 Scope1 2 Normative references .1 3 Principle.1 3.1 General.1 3.2 DNA extraction 2 3.3 DNA quantitation.2 4 General laboratory requirements 2 5 Procedure.2 5.1 Preparation of the test portion.2
8、5.2 DNA extraction/purification4 5.3 Quantitation of the extracted DNA5 5.4 Stability of extracted DNA6 6 Interpretation.6 7 Test report6 Annex A (informative) Methods for DNA extraction7 Annex B (informative) Methods for the quantitation of the extracted DNA34 Bibliography .41 -,-,- ISO 21571:2005(
9、E) iv ISO 2005 All rights reserved Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body in
10、terested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechni
11、cal Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the
12、 technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. ISO 21571 was prepared by the European Committee for Standardization (CEN) Technical Committee CEN/TC 275, Food analy
13、sis Horizontal methods, in collaboration with Technical Committee ISO/TC 34, Food products, in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement). -,-,- ISO 21571:2005(E) ISO 2005 All rights reserved v Introduction The search for genetically modified origin
14、 of ingredients is performed by means of the following successive (or simultaneous) steps. After sample collection, nucleic acids are extracted from the test portion. Extracted nucleic acids can be further purified, simultaneously or after the extraction process. Afterwards, they are quantified (if
15、necessary), diluted (if necessary) and subjected to analytical procedures (such as PCR). These steps are detailed in this and the following International Standards: ISO 21568, Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Sampling. ISO 21569,
16、 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Qualitative nucleic acid based methods. ISO 21570, Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods.
17、 Further information about definitions and general items involving the steps cited above are collected in: ISO 24276, Foodstuffs Nucleic acid based methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions. The International Org
18、anization for Standardization (ISO) draws attention to the fact that it is claimed that compliance with this document may involve the use of a patent concerning the silica-based extraction method (No. EP 0389063/USP 5,234,809) given in Clause A.4. ISO takes no position concerning the evidence, valid
19、ity and scope of this patent right. The holder of this patent right has assured the ISO that he/she is willing to negotiate licences under reasonable and non-discriminatory terms and conditions with applicants throughout the world. In this respect, the statement of the holder of this patent right is
20、 registered with ISO. Information may be obtained from: Jean Deleforge, BioMrieux Chemin de lOrme, 69280 Marcy-ltoile, France. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights other than those identified above. ISO shall not be held
21、 responsible for identifying any or all such patent rights. -,-,- INTERNATIONAL STANDARD ISO 21571:2005(E) ISO 2005 All rights reserved 1 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction 1 Scope This International Standar
22、d provides general requirements and specific methods for DNA extraction/purification and quantitation. These methods are described in Annexes A and B. This International Standard has been established for food matrices, but could also be applicable to other matrices, such as grains and feed. It has b
23、een designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods. 2 Normative references The following referenced documents are indispensable for the application of this document. Fo
24、r dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 24276:1), Foodstuffs Nucleic acid based methods of analysis for the detection of genetically modified organisms and derived products Gener
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