PGRs对愈伤成分种类及其含量的影响.docx

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1、PCRS对*仿油3共有效成分的影看Effectofp1.antsg11thregu1.atorsandIigh1.onthv(routhb1.oyntbeiofadhecompoundsInDictammisdasycarpusTUrCzOdIgcu1.turesO1.KKuniMic(*1MI).fraine1.1.onc(MM),IimoninIrtMHtDk1.ammnv(StNt)目的，1. MRf含仿生氏HUZH资态现成的影响,分切找公优配方.2. PGR，金仿中成分种类24大分Ia侑影响,分别拉世优配方.3. 光照时影侪形.6促成.生长和活性成分/Ift的比咱内M(Mft*ftW为

2、X0tEfSM.5t三j1.,KT工OnISNAA1.生火力It饰生长、海gt成分ISUM至1.*2.4-D.IA,IBA.NAA.-NOA.TIBA,4PAK1.t1.taiOSKT口.O生长率KT.BAKZTsTOZ.HCPPUIADPUOftM*054衿分裂点,20me,1.24D(6蛆试验,施处理，收.I%(U2次)H朴培脩携煌纨维代3次？取样恻定.I6hftffiSHMMh念伤图片颜色和盛地)、2DW,Gk成分种炎和JThtKIi目的I月时分裂卡对必仿旗地.闻色.1.K成分加类和合以算彰响.3.W*M*力率hMSQ5分裂去包0WW1.2.41)MdGOme1.ZADKINAAsinI

3、ZONAA2.4DaIftAQWfNAA粕2.4D压少=不加.111NAAffift/方案2，MS*05KT2Qmg1.,2.4-1)*2Xm1.14-H2.w取wihr*amAnMiMrmvwmkM/N贝色.吩砰的IK快妁产牛，1批找少(thePa1.Cye1.1.ow.EoCrfrui1.c,growingca1.1.uBUa1.1.yhau1.owcub1.i1.yofa1.ka1.oidSyntbeM)2.ABi任影伤支坡叶地/仆成j促进或分种丈及含植包卯M生匕.X2.4-DI优避金伪形成和生KWtt11fe.但和制成分士类利含小Effectofp1.antsrowthregu1.at

4、orsand1.ighton(hegrowthImdbiosynthesiso(activecompoundsinDic1.aniiiusdasycarpusTurcz.ca1.1.uscu1.turesQuindineu1.U1.dvAbstractIn(hepresentedwork,thecf1.ec1.ofp1.antgrowthrcu1.atoc*.inc1.uding05mg,1.Ievdof7auxins(2.4-1).IAA.IBA.NAA.B-NOA.TIBA.4-CPA)and2.0Ina1.k、U1.of6Cyiokintns(K)NBAP.ZEA、TD7、4-CPP1.

5、KU-DPU).wasexaminedfx,Ihcirincncconbio11uvigwuthandtheacmub1.inofmciii11j11.y-rc1.cvin1.biictivecnmpcHincis(bicu11one,Fnuine1.1.one,1.imninax1.DiaamninOinca1.1.uscu1.turesofDkumwusdasycarpus(Rutaeene).成分A类和含量是可变的Theresu1.tmUwedI1.ia1.(bev;IneIyadPrOduC1.ionMbiuc(iveCernpCX1.fXbinca1.1.uscu1.tureofD.

6、daxycatpuscanbem1.h(bW.DW.Gi)WegobtainCdKH0.5m。1.KINinCombirnitinnwith2.0m。1.2.4D.Hnwxrvcnthehighestcontent(#ofDiciamniiK(XKmoreimxrtantconstituent,werercco11fcdintheca1.1.uscu1.turedonMSso1.idmediumsupp1.ementedWiIh05mg1.KINincombinationwith2.0IAA.生长*ttInthegroupofauxins,theCombtnaikNiof0.5ng1.XXXa

7、nd2.(hng,1.YYYPrOVidCdthef;IjiteMcu1.tureKroWth(Gi=).Thecn11ccnf11tfknaMofactivePhtodKTniC工人inca1.1.uscu1.tureswassimi1.ar(othawedpsi1.iveCD11eh1.inuthecy1.nkininsandnegativeComHii1.innwithauxins.元*Oufresu1.tssuggestIhMIighiregu1.ateIifnOn1.nSax1.dicamnnbwsyiMbcsisandhcdiffere1.a!HnOatuscfca1.1.u%cu

8、1.ture%.Quaii1.i1.a1.iveCHIg1.iUnof*waxerttmagrowthandhcMxumuktfionofbiy1.ic%CaHUXDie1.annusdtaycarpus.IXiRs.Dur1.;IJ1.1.mneIntroduction-WWh介作用E八作用打JREB含Ir生生EHbMm至smiMm度.裳AWIHM合创WA廉11知的，优Jk是白w11m*o什么白MMH介：Dicitimnux(Iax)WtrpuxTurcz(Rntacv)KcnCoftheimportantmedicina1.p1.antsax1.nativetoEaaAsia.Tbcroo

9、tharkofthep1.antsicd1.edCWTerdMamniinIhCChinesePharmacopoeia,v*hichisudinChineseIraditiona1.mcdmefortheIret1.nCIHofjaundice,cough,rheumatism收KM1.WEC&indgchM现代ISW九Inmodempharmaco1.ogica1.studies,(hep1.anehasbeenreportedtoPIayvariousbio1.ogica1.ro1.esincancer,inf1.ammakn.andobesityinmanydifferedce1.1.

10、Jypcsandtiuc4uxitobeM1.iP1.皿*罐Zrega1.tion.vucu1.arn7Jing.muhctcria1.n(ifungu1.jCtiVie延W*分TheCnCenWUSthcmpcuticimpotunceandbikgc1.ctivitk).Qf1.dtwrpui(I1.KUnIyduecoConUh1.grcmMnotIn1.ofIimonmsanda1.ka1.oids.Among(bcm.1.imonoid,dictamni.Obacunoncadfraxinc1.1.oarcthenoMdcmonMncdrcrescnuvc1i.原料主SBHI于根破坏

11、性Hwcve,QbCybi.IiVeComJXH1.1.Kkof7iaxycarpaarcmain1.yCnncen1.nitedinthehrkanirctivchins1.ofIheWikJp1.antresu1.tinginthepopu1.ationofWikJresourceincraising1.ydec1.inedordisappearedfromitsnatura1.habit,bKHnxonncdcd.代tfcftTheefbrcinvi1.rbnynthesisofbiw(ivcCnmfXHHKkIhnxighp1.anttissuecu1.1.u11:techniquem

12、ayprovideunakc11utivcapproachforso1.vingthisprob1.em代技术研允JXIBiOSyfHhEsumhfrndiffcmp1.antsviz.a1.ka1.oids.f1.avonoid(货Si类)附类、等等IiQ入引文fromt*ttT4Sp1.ants.IY；R、AXttJMt9(A1.tURPIMmw1.hregu1.akareone1.IiienDtiniptv1.aii1.factuu(fee1.ingce1.1.grow1.h.dIYervii1.ia(insofp1.yk1.x11nnecunpxt1.nocrwidepossibi1.

13、itiesofimprovingce1.1.CUhUrCproductivitywithrespecttothcrupciica1.1.yrcMauCanMiIUcm、dU6*少相关生长节控生长和成分含工的液在U(看文”补上)己有奴多学开JR相美瑞九，某某人事允俺提育“,成分ME1.MMf1.1.nI川HnmbMe1.fUK1.a11jo)fxi,xki1.aidantetikruh&xivy(w11u1.ni1.fmwDkurm(isySXrt.X*M1.M；：KxiiH.KimMKimHSM1.UeQGmAnW.d，S:AtIiatnMmMHitiyMditi1.ie*UrHtmt(day

14、rptz.EMbockE.i1.krpvEEKIdc11MiddbyU1.nITanh11MVCnetnIWCeJMhmMnnKdXU.r2t4T-411.3XC(M(BMhX1.QUtO1.itM)JnJIMnmN“bb1.MUviwfu*pc1.fttIMmiV-YcCCJ.C.Km.S.H.Km.M.J.Vang.H.J.Ah.MR.&Par.JH(2015).1.11cmn.Ccmponen1.ct010a1111ftadsCorte.InhtrtsEacciInducedCdkiumardGAVP1.ae匕8MFKKCRE8SneX)P卅即刈GNCC-M3T3UCeW%g*E纯.22

15、12822136.f1.ttK1.W-31.*PNnxWne1.ka1.im1.rHiitam1exoc1.ktatdtfdk1.Mur.a11uxHaixtiwoxxwmiimwHr11*KiiiDirtxntusdnyafp1Uv.fK4JI3XHG)MPeftCCfreajnfi1.cofSfMomyecHZitdcdbydkrni(K.fdftttIMMS*5*Prt*MjMINUHCvKQhNmik*Zbyhierxtmg:Uudctrcuktr)tt)*fcCkr1.exfnnabtn“evea&nnfIirentin1.dmnvkCiNOMmcmh1miMKk4H附5冏力泣IWr

16、MkIttMUB*;-f-daituiaxx*Jfwnndwpfim:rarp*xm1JPndw，fccKihtw11p11nr11wMan4E加“MVWndXE白IM1.BiH1.W%JS*Inpreviousreport,invircu1.turesofD,Ssyca/pu*wasestab1.ishedinM1.ertoPrOVk1.eanefficicmrcgenerauonPIOIOcoI(引用If几白IUftie的中文文”和自己的上一比攵套)白事*少It方面的研究However.contrarytoothermedicina1.p1.ants,theresearchconcerni

17、ngtheinf1.uenceofp1.umgrowthreuho5CHMhCMiuctionofSeCandafymetabo1.itesarcratherscarceinvitroiiica!1.u%cu1.(ure%ofD.daxycarjnts.本文开JW研究内ThC建imofthepcr%cntc1.Xnrdwaxtoex1.imatetheinf1.uenceofdifferentcombinationsofp1.antgrowthreg1.u(ocsonbiomassPOWthandtheaccumu1.ationof1.imonoid*dic(amnine.obacuHKand

18、fruxinc1.1.oc.themostdcnonsraic1.rcscntativecompoundsinD.dayrknnvkdge.thisisI1.icfirtsuchcnfuhenMvestudyonbiomussgrowthUndtheuccmu1.aiionOfbkxactivccompoundsinvitroinca1.1.uscu1.tures“D.diuyarpus.Materia1.sandmethods*ttJR始念AK方是MSaHUmg1.K20m矶,2*DP1.antmateria1.sThercviouUyCStabnShCdca1.1.usCUkUrCSofD

19、iciannuidascafpuigrownonMSndiumcontaining0.5ng1.kinetin(KIN).2.0mg1.D-Dichkmptienuxyaceticacidand3.0%w/vsucrosewereusedasaMX1.nxofp1.antmacria1.andsubchuredat30daysinwnnk.J1一文/鳍票，EffectoPGRrnnca1.hisgrowthandhkynthc%i%ofhMactieCCmPm1.nd芥Inxxcrtocva1.ii；uctheCfT(XKofauximnca1.1.uxgr1.handthe4ccumu1.a

20、iHiofbio11c(iveCOmP(Xi1.XkinDietannw&dyearpu、ca1.1.uscu1.ture,theca1.1.uswereiubaicdonMSsdidmediumuppk11ven(edwith0.511g1.KINinOfnbiiustxxnw1.1.2.hng,1.1.eve1.erf2.4-1.)v1.1B.N.PM)A,TIB,4-(,P,respective1.y.The1.1.ucrwnonMSmediasupp1.cmcntc.5mjj1.KINm1.yi1.ho11no11e-frccwasconsideredascomrcMMimp1.cs

21、AiKiIogousIy.thecxpcrncnwasPCffDmWd10dctc11mtheeffectofcyiokimnsonca1.1.usgru,1.handIheA.-cuinu1.a1.ionofbiuactiveCUmpoUnihuDiciannivicinycarpuica1.1.ucu1.tures.I1.ieca1.1.usWCEincuhatc1.OnMSso1.idmediumUippicmcntcd0.5mg1.1.eve1.OfK1.N、BAP、力1.A、TDZ.4-CPP1.U1.3-DPUinmbiionwt2.0ng,1.2.4-D.respective1

22、y.Theca1.1.usgrownonMSmediasup1.encdwhh2.0mg,24-Don1.yandhormone-freewereconsideredascontro1.%anp1.es.A1.1.experimentsurrcconductedusingMSso1.idmediumOOmaining3.0%w/vscrscand0.7%My邮ar.pHwas刈Ift1.Cdto5.8prior10autoc1.avinga1.I2X?tbf30mn.theIbCrmMyunstab1.eIKiRs1.T1.BA,Zb1.,I)Z.4Xr,PU,B-DPU1.wereudde

23、dtoUiePCVV)DU3yHU1.OCia、Nmediumbysd(元强K8Mmo1.m:,.PhiIipsIW35WngrCSeCMk11ps.Eachca1.1.ustissue(rownfor30d)of0.2gfreshweight(0.04gDW)wasinitia1.incutxcdonI-Jnicyeff1.ak.v*ith30rep1.icas(6JK)per(rcatncntaid2次Andcchca1.1.usIkWCwasfttt-*hnMi*jkjj11iM11nnni11x*J11wwn*eJrnwnZutojrvwwdmJgktwfnn卬Eo1.ihth/h*d

24、HedMIhePMfttufAikUiruIiiet1.*mef“妙bkrImbc.*11e3Urx*I1.aaitnfrrvdnnPrrAFZJhEirMmhi1.Jne1.thewvis”Utrmir*MEJo11jcpnd1.1.RftttIrvfdir1./icrcJwd1.ih?Iroi*wcrvcwk01.1HCInu1.vc1miIUf1.ttcM11WZAwdrtrTTairMtKXicf1.NirdryuwnsGrowthindex*(WeiShIX-Weigh(.aIWetghaa.Cai1.iwen*drieda60X?for48t.优化个阻力In(henextpanof

25、thestudy,(heca1.1.uswwinocu1.atedonMS11biutionuithdicrcnCemeVnuauonofauxinandcytokininptovidcdIhcbcMIcSUIginICtTn4ofcontent.EffectOr1.iKhtonCaH1.IXgm11handhixnthcixofactiveccimpnunc1.xTbcoi1.Iuswaxincuburte1.ofI6h,daywith1.ightsintensityof88+8UmO1.m2s,at252C.Ca1.1.usfreshweightanddryWCigh1.WCreeva1.

26、uatedacvrditoabovedescribedmethods.QtiantitMtiveJ1.nn1.y6f(H,tirCCE1.MX1.IHR91.1.tBX*ExtractsPrePUnHionundquaniita1.ivcHP1.Cana1.ysesofactiveComPm1.1.XJswerecffrncd.accordingtothePnXxdUrEdCScfibCdprevious1.y9t己*fcfc*1耳全的*Ixrthequami1.ivcana1.yxixofhi(urtiwSmpoMIXiS,1.OgofCadIPaWdercdO1.1.1.UAan1.rH1

27、HneMKJtr*mtUraMt1.意方法411RPJiP1.C对上测定门弊皮中3成分的公0一，Mtei.FdfWik1.p1.antsmutcri1.Waf1.11tmixedwith25m1.chann1.water(75:25,vv)andcxt11c1.ei1.wnIimcvbybeat-ref1.uxingfor2h.Tbcextractionso1.utionsWCrCcombinedundfi1.tratedIhmygh0.22mnc11br3ie.AtIerrecoveringeIiiaxiu1.IheretKIUaIaquexsWa、extractedIwoIinietby

28、IOin1.CH2CI2.1.,hetheCH2C12e.vtrac1.w=、ca3pora1.edtodrynessuungru1.an,evatrakwa1.amuximumtemperatureof451?IjHiI1.ThCcracsWerCdko卜CdviihIOm1.methano1.beforeHP1.Cana)ysikSa11katu1.ysi%waspcrft*medonaKronuisi1.100CISe4uxu(250nn4.6mm.511)arx1.injectionvo1.umewa201.persamp1.eThemobi1.ePhacwnmdhan1.:H20(6

29、0:40.vv)a1.af1.owra1.eof1.Om1.min.Thedetectionwave1.engthandCdwnnICmPCm1.iHCWaSa236nmand35C.fCsp,hve1.y.A1.1.referenceMandarihwerePUrChaydfmmE1.Iiona1.inMituiefbrIheSmm1.CfPturnwEg1.adb)ogica1.ProdUe(.Thecocntraonsofxx.Inam1.csWCredecmincdbytheexterna1.Mandard11etMxJ.Iatanna1.)sA1.1.experimenta1.dat

30、aWCrCStatiMicaIIyaa1.yrodbyonewayana1.ysisofvariance(ANOVA)usingthePrV1.teCICd1.cast-MgncxHdi11cvncc(1.SDtestPrSfc).Resu1.tsanddksionEmoau.hksonca1.1.ugrowthandbioyntH,s1.ofactivecompound、，INUtHYTheeffectofdiI1.erentauxinsonIhebi。Inagruthanddiebxxr(nam1.en(inDidannUSvca1.1.uscu1.tureswen:pre5tcdinFi

31、g.I.Cu1.1.usgrowthandtheconcentrationofbioactivccompoundsprovedtobeMrongiyaffectedbythetype4amountofIbCPGRsIIPP1.ied.AmongthephyichonnoiKtested,thehighestdumninca1.ka1.oidCon1.Cn1.waxacx:vdOnMSwi1.dmediumcmbuaticn0.5ng1.K!Nad1.0mg1.1AA.加入某J*分不俺MA什么但俺整什么Theadditionof2Omg1.auxinshadnosignificanteffect

32、onca1.1.usgroshbutSignif1.candyincrvcdtheaccmu)utionof*content.The7nntn1.andvyn1.he1.icauxinscommon1.yIIvdinVitm、showedconMt1.crab1.cCffEnbiomas、growthandtheaccumu1.ationofRhiactivecompoundsinDkMfinutdaSVCa，PUgca1.1.uscu1.tures产IwS和含囊受It料类和IMt的解，TheypcMdproductionofactiveCOmPOwkiSWCrCdirect1.yU1.I1.

33、vCdbythetypeOfPhytohnrn1.nnCucdandiCxmcrntratinn(没做,衣履)inIhegrowthmedium.hwasdcmiincdthucauxins(2.4-D)addedtoMSmodiuaintheconcentrationof0.5mg1.genera1.1.yHthibi1.edIhcpnductM11of.M比，1.ftT*auxins仿生长AcomparisonOftheGiva1.uesf11rhiom%xcsgrownnmediasupp1.ementedwith2.0mg.,1.QUXi1.Hshowedthathe(cstcdcom

34、poundsMimu1.atcdthegrowthofIheca1.1.us.exceptforIAA.IBA.andNAA.优的Inhserteofcxpcrincu.(hefas1.e1.岁IM1.h(Gii力ax1.Ihehihes1.p111.uctiunc(*r11jgdrywci*hwereachievedontheMS11xxiiumMcntcdwith0.5mg.1.TIB?andO.5mg.1.KIN.f1.11MAWMAACeSdingtorGbHX机,ca31.u%withChrkye1.1.owGrgreenco!w-mwthratecanKynthbiymon;a1.

35、ka1.oids,whi1.ethePakyc1.kw,moremgi1.c.fas-wingCaMUhusua1.1.yhasa1.owcapabi1.ityofa1.ka1.oidynt1.sis.especia1.1.yforttrtt.Tab1.eITheCffeVtof2.0mg1.sxin%COn1.bina1.ienwith0.511,1.KTwthandarumu1.aicnofCXucunonc.Fniiinc1.koe.1.1.moaIn3Dicwnniiicinca1.1.ucu1.ture*AiIXinSFmh*dMWeS240CMt(ib“1.Wec411pMd什DW

36、Cn1.1.mnt(WCUIHUFruxMvbKKI1.ic1.UiiinifMI2.4-D2oIO.2)3o.2.*u,trp*e2IAAj3IBA4NAADftrisv1.ku.t*uf1.5ti-NOAI八TI1.八7MPAEffectofcytokininsonca1.1.usgrowthandbiosjnthcs1.sOractivecompoundsInccm(rustwiththeabovedescribedexperiments,effectofsixnatura1.orsyntheticc,tokinninonca1.1.usgrowthaxibiosynthesisofac

37、ivcCofnPOUndSWCrCPeffbrnMx1.Aintheeaseofs1.dicitwaxohwvc1.thatcyinkininsNigniHGIm1.yinf1.uencedca1.1.usNmWIhandrodcioninca1.1.uscu1.tures.ThCCbsCrVCdcffxisdcpcxJcdonthetypeofPGRsused,aswe1.1.asitCanCCIUraIiOnInthegrowthndium.IbwindetemuedUustexeep,Iokiniiua1.0.5ng,1.significant1.ystimu1.atedthercictinnof*andIntheseCxpcrimcnw1.conditions,aSignifkantincreaseofthecontentoftheinvestigatedMxondaryInC1.abO1.iIC4mca1.1.uswasa1.sonoted.OnIbCotherhand.IbCMScncdwith0.5m,1.ofthe%c1.ectckinininhibitedthe;